LOCUS       sequence-90762-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-sfGFP-DocI
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             748..795
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 atgggtaccg actctctgga attcatcgct tctaaactgg ctctggaagt tctgttccag
       61 ggtccgctgc agcaccacca ccaccaccac ccatggacta gtgctagcag caaaggtgaa
      121 gaactgttta ccggtgttgt tccgattctg gttgaactgg atggtgatgt taatggccac
      181 aaattttcag ttcgtggtga aggcgaaggt gatgcaacca ttggtaaact gaccctgaaa
      241 tttatctgta ccaccggcaa actgccggtt ccgtggccga ccctggttac caccctgacc
      301 tatggtgttc agtgttttag ccgttatccg gatcatatga aacgccacga ttttttcaaa
      361 agcgcaatgc cggaaggtta tgttcaagaa cgtaccatct cctttaaaga cgacggtaaa
      421 tacaaaaccc gtgccgttgt taaatttgaa ggtgataccc tggtgaatcg cattgaactg
      481 aaaggcaccg attttaaaga ggatggtaat atcctgggcc acaaactgga atataatttc
      541 aatagccaca acgtgtatat caccgcagac aaacagaaaa atggcatcaa agccaatttt
      601 accgtgcgcc ataatgttga agatggtagc gtgcagctgg cagatcatta tcagcagaat
      661 accccgattg gtgatggtcc ggttctgctg ccggataatc attatctgag cacccagacc
      721 gttctgagca aagatccgaa tgaaaaacgt gatcatatgg tgctgcatga gtatgttaat
      781 gcagcaggta ttacccatgg tatggatgag ctgtataaga aagtggtacc tggtactcct
      841 tctactaaat tatacggcga cgtcaatgat gacggaaaag ttaactcaac tgacgctgta
      901 gcattgaaga gatatgtttt gagatcaggt ataagcatca acactgacaa tgccgatttg
      961 aatgaagacg gcagagttaa ttcaactgac ttaggaattt tgaagagata tattctcaaa
     1021 gaaatagata cattgccgta caagaactaa
//
