LOCUS       sequence-90764-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..80
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             102..782
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc aagaagttcg
       61 agtccatctc caaggaggag ggggatccac cggtcgccac catgagtgcg attaagccag
      121 acatgaagat caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg
      181 gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg
      241 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg
      301 tattcgccaa atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt
      361 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca
      421 taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg
      481 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt
      541 atgtgcgtga tggagtgctg acgggtgata ttcatatggc tttgttgctt gaaggaaatg
      601 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac
      661 caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca
      721 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat
      781 aa
//
