LOCUS       sequence-90768-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: tdminiSOG-N1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..423
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             451..771
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgga gaagagcttc
      121 gtgatcaccg acccccgcct gcccgacaac cccatcatct tcgccagcga cggcttcctg
      181 gagctgaccg agtacagcag agaggagatc ctgggccgca acggccgctt cctgcagggc
      241 cccgagaccg accaggccac cgtgcagaag atccgcgacg ccatccgcga ccagcgcgag
      301 atcaccgtgc agctgatcaa ctacaccaag agcggcaaga agttctggaa cctgctgcac
      361 ctgcagccca tgcgcgacca gaagggcgag ctgcagtact tcatcggcgt gcagctggac
      421 ggctctggtg gcgggagtgg tggcgggtcc atggagaaaa gtttcgtgat aactgatcca
      481 cggctgccag acaatcccat catcttcgca tccgatggct tcctggagct gaccgagtat
      541 tccagagagg agatcctggg ccgcaatggc cgctttctgc agggaccaga gacagaccag
      601 gccacagtgc agaagattcg cgatgccatt agagatcagc gcgagattac cgtgcagctg
      661 ataaactaca caaaaagcgg gaagaaattc tggaacctcc tgcacctcca gcccatgagg
      721 gaccagaagg gtgagctcca gtatttcatc ggagtgcagc tggatggata agcggccgcg
      781 actctagatc ataatcagcc ataccacatt
//
