LOCUS       sequence-90771-e        1272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Detection construct to label dockerin type I containing fusion 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-StrepII-TagRFP-CohI
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgtggtctc acccgcagtt cgaaaaagtt tctaaaggtg aagaactgat caaagaaaac
       61 atgcacatga aactgtacat ggaaggtact gttaacaacc accacttcaa atgcacctct
      121 gaaggtgaag gtaaaccgta cgaaggtact cagaccatgc gtatcaaagt tgttgaaggt
      181 ggtccgctgc cgttcgcttt cgacatcctg gctacctctt tcatgtacgg ttctcgtacc
      241 ttcatcaacc acacccaggg tatcccggac ttcttcaaac agtctttccc ggaaggtttc
      301 acctgggaac gtgttaccac ctacgaagac ggtggtgttc tgaccgctac ccaggacacc
      361 tctctgcaag acggttgcct gatctacaac gttaaaatcc gtggtgttaa cttcccgtct
      421 aacggtccgg ttatgcagaa aaaaaccctg ggttgggaag ctaacaccga aatgctgtac
      481 ccggctgacg gtggtctgga aggtcgttct gacatggctc tgaaactggt tggtggtggt
      541 cacctgatct gcaacttcaa aaccacctac cgttctaaaa aaccggctaa aaacctgaaa
      601 atgccgggtg tttactacgt tgaccaccgt ctggaacgta tcaaagaagc tgacaaagaa
      661 acctacgttg aacagcacga agttgctgtt gctcgttact gcgacctgcc gtctaaactg
      721 ggtcacaaac tgaacggcag tgtagtacca tcaacacagc ctgtaacaac accacctgca
      781 acaacaaaac cacctgcaac aacaataccg ccgtcagatg atccgaatgc aggatccgac
      841 ggtgtggtag tagaaattgg caaagttacg ggatctgttg gaactacagt tgaaatacct
      901 gtatatttca gaggagttcc atccaaagga atagcaaact gcgactttgt gttcagatat
      961 gatccgaatg tattggaaat tatagggata gatcccggag acataatagt tgacccgaat
     1021 cctaccaaga gctttgatac tgcaatatat cctgacagaa agataatagt attcctgttt
     1081 gcggaagaca gcggaacagg agcgtatgca ataactaaag acggagtatt tgcaaaaata
     1141 agagcaactg taaaatcaag tgctccgggc tatattactt tcgacgaagt aggtggattt
     1201 gcagataatg acctggtaga acagaaggta tcatttatag acggtggtgt taacgttggc
     1261 aatgcaacat aa
//
