LOCUS       sequence-90773-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probing molecule to perform single molecule force spectroscopy 
            experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-CBM-CohI
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtaccg actctctgga attcatcgct tctaaactgg ctctggaagt tctgttccag
       61 ggtccgctgc agcaccacca ccaccaccac ccatggacta gtgctagcat gtgcaataca
      121 gtatcaggca atttgaaggt tgaattctac aacagcaatc cttcagatac tactaactca
      181 atcaatcctc agttcaaggt tactaatacc ggaagcagtg caattgattt gtccaaactc
      241 acattgagat attattatac agtagacgga cagaaagatc agaccttctg gtgtgaccat
      301 gctgcaataa tcggcagtaa cggcagctac aacggaatta cttcaaatgt aaaaggaaca
      361 tttgtaaaaa tgagttcctc aacaaataac gcagacacct accttgaaat aagctttaca
      421 ggcggaactc ttgaaccggg tgcacatgtt cagatacaag gtagatttgc aaagaatgac
      481 tggagtaact atacacagtc aaatgactac tcattcaagt ctgcttcaca gtttgttgaa
      541 tgggatcagg taacagcata cttgaacggt gttcttgtat ggggtaaaga acccggtggc
      601 agtgtagtac catcaacaca gcctgtaaca acaccacctg caacaacaaa accacctgca
      661 acaacaatac cgccgtcaga tgatccgaat gcaggatccg acggtgtggt agtagaaatt
      721 ggcaaagtta cgggatctgt tggaactaca gttgaaatac ctgtatattt cagaggagtt
      781 ccatccaaag gaatagcaaa ctgcgacttt gtgttcagat atgatccgaa tgtattggaa
      841 attataggga tagatcccgg agacataata gttgacccga atcctaccaa gagctttgat
      901 actgcaatat atcctgacag aaagataata gtattcctgt ttgcggaaga cagcggaaca
      961 ggagcgtatg caataactaa agacggagta tttgcaaaaa taagagcaac tgtaaaatca
     1021 agtgctccgg gctatattac tttcgacgaa gtaggtggat ttgcagataa tgacctggta
     1081 gaacagaagg tatcatttat agacggtggt gttaacgttg gcaatgcaac ataa
//
