LOCUS       sequence-90777-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-Spec(x2)-DocI
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtaccg actctctgga attcatcgct tctaaactgg ctctggaagt tctgttccag
       61 ggtccgctgc agcaccacca ccaccaccac ccatggacta gtgctagccg tgctaaactg
      121 aacgaatctc accgtctgca ccagttcttc cgtgacatgg acgacgaaga atcttggatc
      181 aaagaaaaaa aactgctggt ttcttctgaa gactacggtc gtgacctgac cggtgttcag
      241 aacctgcgta aaaaacacaa acgtctggaa gctgaactgg ctgctcacga accggctatc
      301 cagggtgttc tggacaccgg taaaaaactg tctgacgaca acaccatcgg taaagaagaa
      361 atccagcagc gtctggctca gttcgttgac cactggaaag aactgaaaca gctggctgct
      421 gctcgtggtc agcgtctgga agaatctctg gaatacggta gcggtagcgg ttcacgtgct
      481 aaactgaacg aatctcaccg tctgcaccag ttcttccgtg acatggacga cgaagaatct
      541 tggatcaaag aaaaaaaact gctggtttct tctgaagact acggtcgtga cctgaccggt
      601 gttcagaacc tgcgtaaaaa acacaaacgt ctggaagctg aactggctgc tcacgaaccg
      661 gctatccagg gtgttctgga caccggtaaa aaactgtctg acgacaacac catcggtaaa
      721 gaagaaatcc agcagcgtct ggctcagttc gttgaccact ggaaagaact gaaacagctg
      781 gctgctgctc gtggtcagcg tctggaagaa tctctggaat ataaagtggt acctggtact
      841 ccttctacta aattatacgg cgacgtcaat gatgacggaa aagttaactc aactgacgct
      901 gtagcattga agagatatgt tttgagatca ggtataagca tcaacactga caatgccgat
      961 ttgaatgaag acggcagagt taattcaact gacttaggaa ttttgaagag atatattctc
     1021 aaagaaatag atacattgcc gtacaagaac taa
//
