LOCUS       sequence-90781-e        1518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-10FNIII(x4)-DocI
FEATURES             Location/Qualifiers
     source          1..1518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtaccg actctctgga attcatcgct tctaaactgg ctctggaagt tctgttccag
       61 ggtccgctgc agcaccacca ccaccaccac ccatggacta gtgctagcgt tagtgatgtt
      121 ccgcgtgatc tggaagttgt tgcagcaacc ccgaccagcc tgctgattag ctgggatgca
      181 ccggcagtta ccgttcgtta ttatcgtatt acctatggtg aaaccggtgg taatagtccg
      241 gttcaagaat ttaccgttcc gggtagcaaa agcaccgcaa ccattagcgg tctgaaaccg
      301 ggtgttgatt acaccattac cgtttatgcc gttaccggtc gtggtgattc accggcaagc
      361 aacaaaccga ttagcattaa ctatcgtacc ggtagcggta gtggtagcgt ttcagatgtg
      421 cctcgcgacc tggaagtggt ggctgccaca ccgacctcac tgctgatctc atgggatgcc
      481 cctgccgtga ccgtgcgcta ttatcgcatc acatatggcg agacaggtgg caattcacct
      541 gtgcaagaat tcacagttcc tggttcaaaa agtaccgcca caatttctgg cctgaaacct
      601 ggcgtggatt acacaatcac agtgtatgca gtgacaggtc gcggtgatag tccggcaagt
      661 tcaaaaccga tttcaatcaa ttatcgcacc ggctctggct ctggctctgt tagtgatgtt
      721 ccgcgtgatc tggaagttgt tgcagcaacc ccgaccagcc tgctgattag ctgggatgca
      781 ccggcagtta ccgttcgtta ttatcgtatt acctatggtg aaaccggtgg taatagtccg
      841 gttcaagaat ttaccgttcc gggtagcaaa agcaccgcaa ccattagcgg tctgaaaccg
      901 ggtgttgatt acaccattac cgtttatgcc gttaccggtc gtggtgattc accggcaagc
      961 aacaaaccga ttagcattaa ctatcgtacc ggtagcggta gtggtagcgt ttcagatgtg
     1021 cctcgcgacc tggaagtggt ggctgccaca ccgacctcac tgctgatctc atgggatgcc
     1081 cctgccgtga ccgtgcgcta ttatcgcatc acatatggcg agacaggtgg caattcacct
     1141 gtgcaagaat tcacagttcc tggttcaaaa agtaccgcca caatttctgg cctgaaacct
     1201 ggcgtggatt acacaatcac agtgtatgca gtgacaggtc gcggtgatag tccggcaagt
     1261 tcaaaaccga tttcaatcaa tcgcactaaa gtggtacctg gtactccttc tactaaatta
     1321 tacggcgacg tcaatgatga cggaaaagtt aactcaactg acgctgtagc attgaagaga
     1381 tatgttttga gatcaggtat aagcatcaac actgacaatg ccgatttgaa tgaagacggc
     1441 agagttaatt caactgactt aggaattttg aagagatata ttctcaaaga aatagataca
     1501 ttgccgtaca agaactaa
//
