LOCUS       sequence-90790-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mWasabi-N1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..815
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      121 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca
      181 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca
      241 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc
      301 tgaccaccgc gttcagttac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca
      361 actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg
      421 acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg
      481 agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag aaggagacca
      541 ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg
      601 tcaagatgaa gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct
      661 acagggccaa gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga
      721 tcctgaacca cgacaaggac tacaacaagg tgaccgttta cgagatcgcc gtggcccgca
      781 actccaccga cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
      841 agccatacca catttgtaga ggtt
//
