LOCUS       sequence-90793-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 493, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZsGreen1-N1
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3..55
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..764
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
       61 tcgccaccat ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca
      121 tggagggctg cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct
      181 tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg
      241 aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg
      301 acatcgtcga ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc
      361 tgttcgagga cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga
      421 actgcatgta ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga
      481 tgaagaagat gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc
      541 agggcatctt gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc
      601 gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc
      661 acttcatcca gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc
      721 acctgaccga gcacgccatc gcctccggct ccgccttgcc ctgagcggcc gcgactctag
      781 atcataatca gccataccac att
//
