LOCUS       sequence-90795-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4b-N1
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..789
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagtgcgatt
      121 aagccagaca tgaggatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
      181 atcgacggag atggtacagg caagccttat gagggaaaac agaccatgga tcttgaagtc
      241 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc
      301 aacagggtat tcgtgaaata tccagacaac atacaagact attttaagca gtcgtttcct
      361 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caatgccaga
      421 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
      481 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
      541 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattg agatggcttt gttgcttgaa
      601 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
      661 aagttaccag gcgcccactt tgtggaccac gccattgaga ttttaagcca tgacaaagat
      721 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
      781 agacgataag cggccgcgac tctagatcat aatcagccat accacat
//
