LOCUS       sequence-90799-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: YPet-N1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(105..107,108..110,111..824)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(411..430)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaaaggcg
      121 aagagctgtt caccggcgtg gtgcccatcc tggtggagct ggacggcgac gtgaacggcc
      181 acaagttcag cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga
      241 agctgctgtg caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctgg
      301 gctacggcgt gcagtgcttc gcccggtacc ccgaccacat gaagcagcac gacttcttca
      361 agagcgccat gcccgagggc tacgtgcagg agcggaccat cttcttcaag gacgacggca
      421 actacaagac ccgggccgag gtgaagttcg agggcgacac cctggtgaac cggatcgagc
      481 tgaagggcat cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact
      541 acaacagcca caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact
      601 tcaagatccg gcacaacatc gaggacggcg gcgtgcagct ggccgaccac taccagcaga
      661 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccaga
      721 gcgccctgtt caaggacccc aacgagaagc gggaccacat ggtgctgctg gagttcctga
      781 ccgccgccgg catcaccgag ggcatgaacg agctctataa gtaaagcggc cgcgactcta
      841 gatcataatc agccatacca catt
//
