LOCUS       sequence-90804-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of an EosFP variant, mEos2 (a photoconvertible 
            fluorescent protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: pRSETa mEos2
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..98
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(102..110,111..125)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             141..821
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(884..902)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      898..945
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc
       61 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg
      121 ataaggatcg atggggatcc atgagtgcga ttaagccaga catgaagatc aaactccgta
      181 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
      241 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
      301 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca
      361 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
      421 ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt
      481 tctataataa agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga
      541 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
      601 cgggtgatat tcatatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
      661 gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc
      721 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
      781 ctgttgctca ttctggattg cctgacaatg ccagacgata agaattcgaa gcttgatccg
      841 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      901 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      961 atatccggat ctggcgtaat agcga
//
