LOCUS       sequence-90808-e        8913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat alpha2 delta-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, 
            Bauer CS, Pratt WS, Dolphin AC
  TITLE     The alpha2delta subunits of voltage-gated calcium channels form 
            GPI-anchored proteins, a posttranslational modification essential 
            for function.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1654-9. doi: 
            10.1073/pnas.0908735107. Epub 2010 Jan 4.
  PUBMED    20080692
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0908735107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-26- 26"; volume: "107"; issue: "4"; pages: "1654-9"
COMMENT     Sequence Label: Rat Alpha2 delta-1 pMT2
FEATURES             Location/Qualifiers
     source          1..8913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4961..5524
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    5712..5846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5749..5768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5803..5822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6519..6536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6690..7278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6770..6789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7449..8240,8241..8309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8072..8091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8310..8414)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8482..8500
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8538..8560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8660..8679
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8873..8895
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8887..8904
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8888..8904
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attcctcgag
     1081 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattct gcagatcgcg
     1141 aagatggctg ctggctgcct gctggccttg actctgacac ttttccaatc ttggctgatc
     1201 ggcccctcga gcgaggagcc cttcccttcg cccgtcacta tcaagtcatg ggtggataaa
     1261 atgcaggaag accttgtcac actggcaaag acagcaagtg gagtgactca gcttgctgac
     1321 atttatgaaa aataccaaga tttgtatact gtggagccaa ataatgcacg ccaactggtt
     1381 gaaattgcag ctagagacat tgagaaactt ctgagcaaca gatctaaagc cctggtgcgc
     1441 ctggctatgg aagcagagaa agtccaagcc gcccaccaat ggagggaaga ttttgcaagc
     1501 aacgaagttg tctactacaa cgctaaagac gatcttgatc ctgaaagaaa tgagagtgag
     1561 tcaggcagcc aacggattaa acctgttttc atcgaagacg ctaattttgg acgccagata
     1621 tcctatcaac atgcagcggt ccatattccc acggacatct atgagggctc aaccatagtg
     1681 ttaaacgaac tcaactggac aagtgcctta gatgaagttt tcaaaagaaa tcgagatgaa
     1741 gaccctacat tgctgtggca agtgtttggc agcgccaccg gcctggcccg atattatcca
     1801 gcttctccgt gggtggataa cagcagaact ccaaacaaga tcgatctcta cgatgtacgc
     1861 agaagaccat ggtacatcca gggagctgca tcccccaaag acatgctcat tctggtggac
     1921 gtgagtggaa gtgtgagcgg attgactctg aaactcatcc ggacatctgt ctctgagatg
     1981 ttagaaaccc tttccgatga tgatttcgtg aatgtagctt catttaacag caatgctcag
     2041 gatgtgagtt gtttccagca cctggttcaa gcgaatgtaa ggaacaagaa ggtgctgaag
     2101 gatgcggtga ataacatcac agcaaagggg ataacagatt acaagaaagg ctttagcttc
     2161 gcgtttgaac agctacttaa ttataatgtt tccagagcca attgtaataa gatcatcatg
     2221 ttattcacgg atggaggaga agagagagcc caggagatat ttgccaagta caataaagac
     2281 aaaaaagtcc gcgtgtttac attttctgtt ggtcaacata attatgacag aggacctatt
     2341 cagtggatgg cttgtgaaaa caaaggttat tattatgaga ttccctctat tggtgcaata
     2401 agaatcaata ctcaggaata tctagatgtt ctgggaagac caatggtttt agccggggac
     2461 aaagcaaagc aagtccagtg gacaaatgtg tatttggatg ctctggagct gggacttgtc
     2521 attactggaa ctctaccggt cttcaacgtc actggccagt ctgaaaataa gactaacttg
     2581 aagaaccagt tgattcttgg tgtgatgggc gttgatgtgt ctctggaaga tattaagaga
     2641 ttgacaccac gttttacact gtgccccaac ggttactatt ttgcaatcga tcctaatggt
     2701 tatgtcttac tgcatccaaa tcttcagcca aagaacccca aatctcagga gccggtcaca
     2761 ctggattttc tcgatgctga gttagagaat gatattaaag tggagattcg aaataaaatg
     2821 atagacggag aaagtggaga aaaaacattc agaactctgg tcaaatctca agatgagaga
     2881 tatattgaca aaggaaatcg aacatacaca tggacacctg tcaatggcac agattacagt
     2941 ttggccttgg tattgccaac ctacagtttt tactatataa aagccaaaat agaagagaca
     3001 ataactcagg ccagatcaaa aaaggggaaa atgaaggatt cagaaaccct gaagccagac
     3061 aattttgaag aatctggcta cactttcata gcaccaaggg aatactgcaa tgacctgaaa
     3121 ccttcagata ataacactga atttctttta aatttcaatg aatttattga taggaaaact
     3181 ccaaacaacc cttcctgtaa tacagatttg attaatagaa tcttgctgga tgcgggtttt
     3241 acaaatgaac ttgtccaaaa ttattggagt aaacagaaaa atatcaaagg agtgaaggca
     3301 cgctttgtgg tgactgatgg cggaattaca agagtttacc ccaaagaggc tggagaaaac
     3361 tggcaagaga acccagagac gtatgaggac agcttctaca aacggagcct agataacgat
     3421 aactacgttt tcactgcgcc ctacttcaac aaaagtggac ctggtgccta tgaatctgga
     3481 attatggtaa gcaaagctgt agaactgtac atccaaggaa aacttcttaa gcctgcagtt
     3541 gtgggaatta aaattgacgt aaactcctgg atagaaaatt ttaccaaaac ttcaatcagg
     3601 gatccgtgtg ctggtccagt ttgtgactgc aaaagaaaca gtgatgtaat ggactgtgtc
     3661 attctagatg atggtggatt tcttctgatg gccaatcatg atgattacac taatcagatt
     3721 ggacgttttt ttggagagat tgacccgagc atgatgagac acctggttaa tatatcactt
     3781 tatgcattca ataaatcata tgactatcag tctgtgtgtg atccaggggc agcaccaaag
     3841 caaggagcag gacatcgctc agcctatgtg ccatcaatta cagacatact ccagattggc
     3901 tggtgggcca ctgctgccgc ctggtctatt ctccagcagc tactcttgag tttgacattt
     3961 ccacggctcc ttgaggcagt tgaaatggaa gaggatgact tcacagcttc cctgtctaag
     4021 cagagctgca tcacagaaca aactcagtac ttcttcaaga acgatactaa atcattcagt
     4081 ggtttactgg actgtggaaa ctgttccagg atctttcatg ttgagaagct tatgaacacc
     4141 aacctagtgt tcataatggt ggagagcaag ggaacatgtc cgtgtgacac gcggctgctc
     4201 atgcaagcag aacagacttc tgatggtcca gatccttgcg acatggttaa gcagcccaga
     4261 taccgaaaag gacctgatgt ctgctttgat aacaatgtgc tggaggatta taccgactgt
     4321 ggtggtgttt ctggattaaa cccttcctta tggtctatct ttggactcca atttatactc
     4381 ctttggctgg tatctggcag cagacactat ctatggtgac cctctaaacc catgatctgc
     4441 aacttaattc catgccctgc caaaacatga gccctcagtt acattaaaat agagcccgtt
     4501 gaagaagctc cacagaacct tagctgggcc tctaccctga tgtaactgtg aggcttagat
     4561 tcctgaccat cgctggctgc atgtcagggt gttggaaact gaaactcgtg tgaacatgct
     4621 gcatcatctg tgtccaacat caaagcaaag ctctactcat tctctgtggg aggtttggga
     4681 gttcgctgtt gcactgttgg agattacgtg gaaaaggctc ccccacgcag gccttatgaa
     4741 aagaagcagt cttgactttg acctagcatt tcaacctgct gcagttttac caagccgaat
     4801 tcctgcagcc cgggggatcc actagatcca tcacactggc ggccgcgtcg acgaattccg
     4861 gggggggggg ggggggggac agctcagggc tgcgatttcg cgccaaactt gacggcaatc
     4921 ctagcgtgaa ggctggtagg attttatccc cgctgccatc atggttcgac cattgaactg
     4981 catcgtcgcc gtgtcccaaa atatggggat tggcaagaac ggagacctac cctggcctcc
     5041 gctcaggaac gagttcaagt acttccaaag aatgaccaca acctcttcag tggaaggtaa
     5101 acagaatctg gtgattatgg gtaggaaaac ctggttctcc attcctgaga agaatcgacc
     5161 tttaaaggac agaattaata tagttctcag tagagaactc aaagaaccac cacgaggagc
     5221 tcattttctt gccaaaagtt tggatgatgc cttaagactt attgaacaac cggaattggc
     5281 aagtaaagta gacatggttt ggatagtcgg aggcagttct gtttaccagg aagccatgaa
     5341 tcaaccaggc cacctcagac tctttgtgac aaggatcatg caggaatttg aaagtgacac
     5401 gtttttccca gaaattgatt tggggaaata taaacttctc ccagaatacc caggcgtcct
     5461 ctctgaggtc caggaggaaa aaggcatcaa gtataagttt gaagtctacg agaagaaaga
     5521 ctaacaggaa gatgctttca agttctctgc tcccctccta aagctatgca ttttttataa
     5581 gaccatggga cttttgctgg ctttagatca taatcagcca taccacattt gtagaggttt
     5641 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5701 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5821 tcaatgtatc ttatcatgtc tggatccccg gccaacggtc tggtgacccg gctgcgagag
     5881 ctcggtgtac ctgagacgcg agtaagccct tgagtcaaag acgtagtcgt tgcaagtccg
     5941 caccaggtac tgatatccca ccaaaaagtg cggcggcggc tggcggtaga ggggccagcg
     6001 tagggtggcc ggggctccgg gggcgaggtc ttccaacata aggcgatgat atccgtagat
     6061 gtacctggac atccaggtga tgccggcggc ggtggtggag gcgcgcggaa agtcgcggac
     6121 gcggttccag atgttgcgca gcggcaaaaa gtgctccatg gtcgggacgc tctggccggt
     6181 gaggcgtgcg cagtcgttga cgctctagac cgtgcaaaag gagagcctgt aagcgggcac
     6241 tcttccgtgg tctggtggat aaattcgcaa gggtatcatg gcggacgacc ggggttcgaa
     6301 ccccggatcc ggccgtccgc cgtgatccat ccggttaccg cccgcgtgtc gaacccaggt
     6361 gtgcgacgtc agacaacggg ggagcgctcc ttttggcttc cttccaggcg cggcggctgc
     6421 tgcgctagct tttttggcga gctcgaatta attctgcatt aatgaatcgg ccaacgcgcg
     6481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     6901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8461 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8521 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     8581 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     8641 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     8701 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     8761 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     8821 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     8881 cgacgttgta aaacgacggc cagtgccaag ctt
//
