LOCUS       sequence-90809-e        8478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse Alpha2 delta-3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, 
            Bauer CS, Pratt WS, Dolphin AC
  TITLE     The alpha2delta subunits of voltage-gated calcium channels form 
            GPI-anchored proteins, a posttranslational modification essential 
            for function.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1654-9. doi: 
            10.1073/pnas.0908735107. Epub 2010 Jan 4.
  PUBMED    20080692
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0908735107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-26- 26"; volume: "107"; issue: "4"; pages: "1654-9"
COMMENT     Sequence Label: Mouse Alpha2 delta-3 pMT2
FEATURES             Location/Qualifiers
     source          1..8478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4526..5089
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    5277..5411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5314..5333)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5368..5387
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6084..6101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6255..6843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6335..6354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7014..7805,7806..7874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7637..7656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7875..7979)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8047..8065
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8103..8125)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8225..8244
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8438..8460
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8452..8469
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8453..8469
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attgcggccg
     1081 ctccagtcgc cgccgccgcc gccgccgcca ggatggccgg gccgggctcg ctgtgctgcg
     1141 cgtcccgggg ggcctcggcg ctcctagcca ccgcgcttct ctacgccgct ctgggggacg
     1201 tggtgcgctc cgagcagcag atcccgctct ccgtagtgaa gctgtgggcc tccgcttttg
     1261 gtggggagat aaaatccatc gctgccaagt actcgggctc ccagcttctg caaaagaaat
     1321 acaaagagta tgagaaggac gttgccattg aagaaatcga cggtctccaa ctggtgaaga
     1381 agttggctaa aatcatggag gagatgttcc acaagaagtc cgaggcagtg aggcgtcttg
     1441 tggaggctgc agaggaggca cacctgaaac atgaatttga tgctgacttg cagtatgaat
     1501 acttcaatgc tgtgctgatc aacgagaggg acaaagacgg gaactttttg gaattgggaa
     1561 aggaattcat cttagccccc aatgaccatt ttaataattt gcctgtgaac atcagtctga
     1621 gtgatgtgca agtgccaacg aacatgtaca acaaagatcc tgccattgtc aatggagtgt
     1681 attggtctga atctctaaac aaagtttttg tggataactt tgatcgggat ccgtctctca
     1741 tatggcagta ctttggaagt gcaaagggct ttttcagaca gtacccaggg attaaatggg
     1801 aaccagatga gaatggagtc attgcctttg actgcaggaa cagaaaatgg tacatccagg
     1861 cagcgacttc tccaaaggat gttgtcattt tggtggacgt cagtgggagc atgaaaggac
     1921 tccgcttgac catcgccaag caaacagtgt cctcaatact ggatactctg ggtgatgatg
     1981 acttcttcaa catcatcacg tataacgaag agcttcacta tgtggaacct tgtctgaacg
     2041 gaacactggt tcaagctgac aggaccaaca aggagcactt cagggagcat ttggacaaac
     2101 tttttgccaa agggattgga atgctggata tcgcactgaa cgaggccttc aacatactga
     2161 gcgatttcaa ccacactgga caaggaagca tttgcagcca ggccatcatg ctcataactg
     2221 atggggcagt ggacacctat gataccatct ttgcaaaata taattggcca gaccgaaagg
     2281 ttcgaatctt tacttacctc attggacggg aggctgcttt tgccgacaat ctcaagtgga
     2341 tggcttgtgc taacaaagga tttttcaccc agatctccac cttggctgat gtgcaggaaa
     2401 atgtcatgga atacctccat gtgcttagcc gacctaaagt cattgaccag gagcatgatg
     2461 tggtgtggac cgaagcgtac attgacagca ccctccctca ggctcaaaag cttgctgatg
     2521 atcagggcct cgtcttgatg accacggtgg ccatgcctgt gtttagtaag cagaacgaaa
     2581 ctaggtcaaa gggcattctt ctgggtgtgg ttggcacaga tgtcccagtt aaagagcttc
     2641 tgaagaccat ccccaaatac aagttaggaa ttcatggtta cgccttcgcc atcacgaata
     2701 atggatatat cctgacgcac ccggagctca ggccactgta tgaagaagga aaaaaacgga
     2761 ggaaacccaa ctacagtagt gtggatctct ctgaagtcga gtgggaagac cgggatgatg
     2821 tgttacgaaa tgccatggta aatcggaaga ctgggaaatt ctccatggaa gtgaagaaga
     2881 ccgtggacaa agggaaacgg gttttggtga tgaccaatga ctactactat acagacatca
     2941 agggtactcc tttcagttta ggtgtggcgc tctccagggg ccatgggaaa tacttcttcc
     3001 gagggaatgt aaccattgaa gaaggcctcc atgacttaga acatcctgac gtgtccttgg
     3061 cagatgaatg gtcctactgc aacactgacc tgcacccaga gcaccgccat ctatctcaac
     3121 tggaagccat taagctctac ctcaaaggca aggagcctct gcttcaatgt gacaaagaat
     3181 tgattcaaga agtccttttt gatgctgtgg tgagcgcccc tattgaagcc tattggacga
     3241 gcctggccct caacaaatct gagaattctg acaagggtgt agaggtcgcc ttcctcggca
     3301 ctcgcacagg cctctcaaga atcaacctgt ttgtgggggc cgaacaactc accaatcagg
     3361 actttctgaa ggctggagac aaagagaaca tttttaatgc cgatcatttc cctctctggt
     3421 acagaagagc tgccgagcag atcgcaggaa gctttgtcta ttccatcccc ttcagcacag
     3481 gaacagtcaa caaaagcaat gtggtgacag caagtacctc catccagctc ctggatgagc
     3541 ggaaatctcc cgtggtggca gctgtaggca ttcagatgaa acttgaattc ttccaaagga
     3601 agttctggac tgccagcaga cagtgtgcct ccctggatgg caaatgctcc ataagctgcg
     3661 atgacgagac tgtgaactgt taccttatag acaataacgg attcattctg gtgtctgaag
     3721 actacacaca gactggagat ttttttggtg aggtggaagg agctgtcatg aacaagttgt
     3781 taacaatggg ttcctttaaa agaataacct tgtacgacta ccaagccatg tgtagagcca
     3841 acaaggagag cagtgacagt gcccatggac ttctggaccc ctataaggcc tttctctctg
     3901 cagccaagtg gataatgacg gaacttgtct tgttcctggt ggagtttaac ctgtgcagtt
     3961 ggtggcactc cgacatgaca gctaaagccc agaaactgaa acagaccctg gaaccttgtg
     4021 atactgaata cccagccttt gtttctgaac gcaccatcaa ggagaccaca gggaacattg
     4081 cttgcgaaga ctgctccaag tccttcgtca tccagcaaat cccgagtagc aatctgttca
     4141 tggtggtggt ggacagtagc tgtctctgtg agtccgtggc tcctataacc atggcaccca
     4201 ttgaaatcag gtataatgaa tcccttaagt gtgaacggtt aaaggctcag aagatcagac
     4261 gacgtccaga atcctgccac ggcttccatc ctgaggagaa tgcaagagag tgtgggggtg
     4321 catcgagtct tcaggcccag gcggccttgc tgctgctgcc cctggtttcg agtctcttct
     4381 caaggtgagc ggccgcacta gtcccgggta cctcgaggaa ttccgggggg gggggggggg
     4441 gggacagctc agggctgcga tttcgcgcca aacttgacgg caatcctagc gtgaaggctg
     4501 gtaggatttt atccccgctg ccatcatggt tcgaccattg aactgcatcg tcgccgtgtc
     4561 ccaaaatatg gggattggca agaacggaga cctaccctgg cctccgctca ggaacgagtt
     4621 caagtacttc caaagaatga ccacaacctc ttcagtggaa ggtaaacaga atctggtgat
     4681 tatgggtagg aaaacctggt tctccattcc tgagaagaat cgacctttaa aggacagaat
     4741 taatatagtt ctcagtagag aactcaaaga accaccacga ggagctcatt ttcttgccaa
     4801 aagtttggat gatgccttaa gacttattga acaaccggaa ttggcaagta aagtagacat
     4861 ggtttggata gtcggaggca gttctgttta ccaggaagcc atgaatcaac caggccacct
     4921 cagactcttt gtgacaagga tcatgcagga atttgaaagt gacacgtttt tcccagaaat
     4981 tgatttgggg aaatataaac ttctcccaga atacccaggc gtcctctctg aggtccagga
     5041 ggaaaaaggc atcaagtata agtttgaagt ctacgagaag aaagactaac aggaagatgc
     5101 tttcaagttc tctgctcccc tcctaaagct atgcattttt tataagacca tgggactttt
     5161 gctggcttta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     5221 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     5281 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5341 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5401 atgtctggat ccccggccaa cggtctggtg acccggctgc gagagctcgg tgtacctgag
     5461 acgcgagtaa gcccttgagt caaagacgta gtcgttgcaa gtccgcacca ggtactgata
     5521 tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc cagcgtaggg tggccggggc
     5581 tccgggggcg aggtcttcca acataaggcg atgatatccg tagatgtacc tggacatcca
     5641 ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg cggacgcggt tccagatgtt
     5701 gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg ccggtgaggc gtgcgcagtc
     5761 gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg ggcactcttc cgtggtctgg
     5821 tggataaatt cgcaagggta tcatggcgga cgaccggggt tcgaaccccg gatccggccg
     5881 tccgccgtga tccatccggt taccgcccgc gtgtcgaacc caggtgtgcg acgtcagaca
     5941 acgggggagc gctccttttg gcttccttcc aggcgcggcg gctgctgcgc tagctttttt
     6001 ggcgagctcg aattaattct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6421 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     6481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6661 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7981 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     8041 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     8101 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     8161 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     8221 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8281 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     8341 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8401 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8461 acggccagtg ccaagctt
//
