LOCUS       sequence-90811-e        8940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of HA tagged rat Alpha2 delta-1 in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Kadurin I, Alvarez-Laviada A, Ng SF, Walker-Gray R, D'Arco M, Fadel 
            MG, Pratt WS, Dolphin AC
  TITLE     Calcium currents are enhanced by alpha2delta-1 lacking its membrane 
            anchor.
  JOURNAL   J Biol Chem. 2012 Sep 28;287(40):33554-66. Epub 2012 Aug 6.
  PUBMED    22869375
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2012-09-28"; volume: "287(40)"; pages: "33554-66. 
            Epub 2012 Aug 6"
COMMENT     Sequence Label: Rat Alpha2 delta-1 HA pMT2
FEATURES             Location/Qualifiers
     source          1..8940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2791..2817
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4988..5551
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    5739..5873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5776..5795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5830..5849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6546..6563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6717..7305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6797..6816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7476..8267,8268..8336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8099..8118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8337..8441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8509..8527
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8565..8587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8687..8706
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8900..8922
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8914..8931
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8915..8931
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attcctcgag
     1081 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattct gcagatcgcg
     1141 aagatggctg ctggctgcct gctggccttg actctgacac ttttccaatc ttggctgatc
     1201 ggcccctcga gcgaggagcc cttcccttcg cccgtcacta tcaagtcatg ggtggataaa
     1261 atgcaggaag accttgtcac actggcaaag acagcaagtg gagtgactca gcttgctgac
     1321 atttatgaaa aataccaaga tttgtatact gtggagccaa ataatgcacg ccaactggtt
     1381 gaaattgcag ctagagacat tgagaaactt ctgagcaaca gatctaaagc cctggtgcgc
     1441 ctggctatgg aagcagagaa agtccaagcc gcccaccaat ggagggaaga ttttgcaagc
     1501 aacgaagttg tctactacaa cgctaaagac gatcttgatc ctgaaagaaa tgagagtgag
     1561 tcaggcagcc aacggattaa acctgttttc atcgaagacg ctaattttgg acgccagata
     1621 tcctatcaac atgcagcggt ccatattccc acggacatct atgagggctc aaccatagtg
     1681 ttaaacgaac tcaactggac aagtgcctta gatgaagttt tcaaaagaaa tcgagatgaa
     1741 gaccctacat tgctgtggca agtgtttggc agcgccaccg gcctggcccg atattatcca
     1801 gcttctccgt gggtggataa cagcagaact ccaaacaaga tcgatctcta cgatgtacgc
     1861 agaagaccat ggtacatcca gggagctgca tcccccaaag acatgctcat tctggtggac
     1921 gtgagtggaa gtgtgagcgg attgactctg aaactcatcc ggacatctgt ctctgagatg
     1981 ttagaaaccc tttccgatga tgatttcgtg aatgtagctt catttaacag caatgctcag
     2041 gatgtgagtt gtttccagca cctggttcaa gcgaatgtaa ggaacaagaa ggtgctgaag
     2101 gatgcggtga ataacatcac agcaaagggg ataacagatt acaagaaagg ctttagcttc
     2161 gcgtttgaac agctacttaa ttataatgtt tccagagcca attgtaataa gatcatcatg
     2221 ttattcacgg atggaggaga agagagagcc caggagatat ttgccaagta caataaagac
     2281 aaaaaagtcc gcgtgtttac attttctgtt ggtcaacata attatgacag aggacctatt
     2341 cagtggatgg cttgtgaaaa caaaggttat tattatgaga ttccctctat tggtgcaata
     2401 agaatcaata ctcaggaata tctagatgtt ctgggaagac caatggtttt agccggggac
     2461 aaagcaaagc aagtccagtg gacaaatgtg tatttggatg ctctggagct gggacttgtc
     2521 attactggaa ctctaccggt cttcaacgtc actggccagt ctgaaaataa gactaacttg
     2581 aagaaccagt tgattcttgg tgtgatgggc gttgatgtgt ctctggaaga tattaagaga
     2641 ttgacaccac gttttacact gtgccccaac ggttactatt ttgcaatcga tcctaatggt
     2701 tatgtcttac tgcatccaaa tcttcagcca aagaacccca aatctcagga gccggtcaca
     2761 ctggattttc tcgatgctga gttagagaat tacccttacg acgtccctga ctacgccgat
     2821 attaaagtgg agattcgaaa taaaatgata gacggagaaa gtggagaaaa aacattcaga
     2881 actctggtca aatctcaaga tgagagatat attgacaaag gaaatcgaac atacacatgg
     2941 acacctgtca atggcacaga ttacagtttg gccttggtat tgccaaccta cagtttttac
     3001 tatataaaag ccaaaataga agagacaata actcaggcca gatcaaaaaa ggggaaaatg
     3061 aaggattcag aaaccctgaa gccagacaat tttgaagaat ctggctacac tttcatagca
     3121 ccaagggaat actgcaatga cctgaaacct tcagataata acactgaatt tcttttaaat
     3181 ttcaatgaat ttattgatag gaaaactcca aacaaccctt cctgtaatac agatttgatt
     3241 aatagaatct tgctggatgc gggttttaca aatgaacttg tccaaaatta ttggagtaaa
     3301 cagaaaaata tcaaaggagt gaaggcacgc tttgtggtga ctgatggcgg aattacaaga
     3361 gtttacccca aagaggctgg agaaaactgg caagagaacc cagagacgta tgaggacagc
     3421 ttctacaaac ggagcctaga taacgataac tacgttttca ctgcgcccta cttcaacaaa
     3481 agtggacctg gtgcctatga atctggaatt atggtaagca aagctgtaga actgtacatc
     3541 caaggaaaac ttcttaagcc tgcagttgtg ggaattaaaa ttgacgtaaa ctcctggata
     3601 gaaaatttta ccaaaacttc aatcagggat ccgtgtgctg gtccagtttg tgactgcaaa
     3661 agaaacagtg atgtaatgga ctgtgtcatt ctagatgatg gtggatttct tctgatggcc
     3721 aatcatgatg attacactaa tcagattgga cgtttttttg gagagattga cccgagcatg
     3781 atgagacacc tggttaatat atcactttat gcattcaata aatcatatga ctatcagtct
     3841 gtgtgtgatc caggggcagc accaaagcaa ggagcaggac atcgctcagc ctatgtgcca
     3901 tcaattacag acatactcca gattggctgg tgggccactg ctgccgcctg gtctattctc
     3961 cagcagctac tcttgagttt gacatttcca cggctccttg aggcagttga aatggaagag
     4021 gatgacttca cagcttccct gtctaagcag agctgcatca cagaacaaac tcagtacttc
     4081 ttcaagaacg atactaaatc attcagtggt ttactggact gtggaaactg ttccaggatc
     4141 tttcatgttg agaagcttat gaacaccaac ctagtgttca taatggtgga gagcaaggga
     4201 acatgtccgt gtgacacgcg gctgctcatg caagcagaac agacttctga tggtccagat
     4261 ccttgcgaca tggttaagca gcccagatac cgaaaaggac ctgatgtctg ctttgataac
     4321 aatgtgctgg aggattatac cgactgtggt ggtgtttctg gattaaaccc ttccttatgg
     4381 tctatctttg gactccaatt tatactcctt tggctggtat ctggcagcag acactatcta
     4441 tggtgaccct ctaaacccat gatctgcaac ttaattccat gccctgccaa aacatgagcc
     4501 ctcagttaca ttaaaataga gcccgttgaa gaagctccac agaaccttag ctgggcctct
     4561 accctgatgt aactgtgagg cttagattcc tgaccatcgc tggctgcatg tcagggtgtt
     4621 ggaaactgaa actcgtgtga acatgctgca tcatctgtgt ccaacatcaa agcaaagctc
     4681 tactcattct ctgtgggagg tttgggagtt cgctgttgca ctgttggaga ttacgtggaa
     4741 aaggctcccc cacgcaggcc ttatgaaaag aagcagtctt gactttgacc tagcatttca
     4801 acctgctgca gttttaccaa gccgaattcc tgcagcccgg gggatccact agatccatca
     4861 cactggcggc cgcgtcgacg aattccgggg gggggggggg gggggacagc tcagggctgc
     4921 gatttcgcgc caaacttgac ggcaatccta gcgtgaaggc tggtaggatt ttatccccgc
     4981 tgccatcatg gttcgaccat tgaactgcat cgtcgccgtg tcccaaaata tggggattgg
     5041 caagaacgga gacctaccct ggcctccgct caggaacgag ttcaagtact tccaaagaat
     5101 gaccacaacc tcttcagtgg aaggtaaaca gaatctggtg attatgggta ggaaaacctg
     5161 gttctccatt cctgagaaga atcgaccttt aaaggacaga attaatatag ttctcagtag
     5221 agaactcaaa gaaccaccac gaggagctca ttttcttgcc aaaagtttgg atgatgcctt
     5281 aagacttatt gaacaaccgg aattggcaag taaagtagac atggtttgga tagtcggagg
     5341 cagttctgtt taccaggaag ccatgaatca accaggccac ctcagactct ttgtgacaag
     5401 gatcatgcag gaatttgaaa gtgacacgtt tttcccagaa attgatttgg ggaaatataa
     5461 acttctccca gaatacccag gcgtcctctc tgaggtccag gaggaaaaag gcatcaagta
     5521 taagtttgaa gtctacgaga agaaagacta acaggaagat gctttcaagt tctctgctcc
     5581 cctcctaaag ctatgcattt tttataagac catgggactt ttgctggctt tagatcataa
     5641 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5701 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5761 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5821 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccccggcc
     5881 aacggtctgg tgacccggct gcgagagctc ggtgtacctg agacgcgagt aagcccttga
     5941 gtcaaagacg tagtcgttgc aagtccgcac caggtactga tatcccacca aaaagtgcgg
     6001 cggcggctgg cggtagaggg gccagcgtag ggtggccggg gctccggggg cgaggtcttc
     6061 caacataagg cgatgatatc cgtagatgta cctggacatc caggtgatgc cggcggcggt
     6121 ggtggaggcg cgcggaaagt cgcggacgcg gttccagatg ttgcgcagcg gcaaaaagtg
     6181 ctccatggtc gggacgctct ggccggtgag gcgtgcgcag tcgttgacgc tctagaccgt
     6241 gcaaaaggag agcctgtaag cgggcactct tccgtggtct ggtggataaa ttcgcaaggg
     6301 tatcatggcg gacgaccggg gttcgaaccc cggatccggc cgtccgccgt gatccatccg
     6361 gttaccgccc gcgtgtcgaa cccaggtgtg cgacgtcaga caacggggga gcgctccttt
     6421 tggcttcctt ccaggcgcgg cggctgctgc gctagctttt ttggcgagct cgaattaatt
     6481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6901 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7141 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8461 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8521 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8581 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8641 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8701 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8761 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8821 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8881 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
//
