LOCUS       sequence-90821-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4b-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..80
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..779
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNAR"
ORIGIN
        1 agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc aagaagttcg
       61 agtccatctc caaggaggag ggggatccac cggtcgccac catggtgagt gcgattaagc
      121 cagacatgag gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
      181 acggagatgg tacaggcaag ccttatgagg gaaaacagac catggatctt gaagtcaaag
      241 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
      301 gggtattcgt gaaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg
      361 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcaat gccagaaacg
      421 acataacaat ggaaggggac actttctata ataaagttcg attttatggt accaactttc
      481 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
      541 tgtatgtgcg tgatggagtg ctgacgggtg atattgagat ggctttgttg cttgaaggaa
      601 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
      661 taccaggcgc ccactttgtg gaccacgcca ttgagatttt aagccatgac aaagattaca
      721 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
//
