LOCUS       sequence-90822-e       12571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit CaV2.2 calcium channels with a mutation (W391) 
            in the alpha-1-interacting domain (AID) in the I-II loop of CaV2.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Leroy J, Richards MW, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies 
            A, Dolphin AC
  TITLE     Interaction via a key tryptophan in the I-II linker of N-type 
            calcium channels is required for beta1 but not for palmitoylated 
            beta2, implicating an additional binding site in the regulation of 
            channel voltage-dependent properties.
  JOURNAL   J Neurosci. 2005 Jul 27;25(30):6984-96.
  PUBMED    16049174
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2005-07-27"; volume: "25(30)"; pages: "6984-96"
COMMENT     Sequence Label: Rabbit Cav 2.2 W391A pMT2
FEATURES             Location/Qualifiers
     source          1..12571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             8619..9182
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    9370..9504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9407..9426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9461..9480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10177..10194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10348..10936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10428..10447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11107..11898,11899..11967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11730..11749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11968..12072)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12140..12158
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12196..12218)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12318..12337
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12531..12553
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12545..12562
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12546..12562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attgcggccg
     1081 cactagtccc gggtacctcg aggaattcct cgagggtacc gggccccccc tcgaggtcga
     1141 agcttgatca tggtccgctt cggggacgag ctgggcggcc gctatggggg cgcgggcggc
     1201 gcggagcggg cccggggcgg cggggccggc ggggctggcg gcccgggccc cggggggctg
     1261 ccgccgggcc agcgggtcct ctacaaacag tcgatcgcgc agcgcgcgcg gaccatggcg
     1321 ctgtacaacc ccatcccggt caagcagaac tgcttcaccg tcaaccgctc gctcttcgtc
     1381 ttcagcgagg acaacgtcgt ccgcaaatac gccaagcgca tcaccgagtg gcctccgttc
     1441 gagtacatga tcctggccac catcatcgcc aactgcatcg tgctggccct ggagcagcac
     1501 ctccctgacg gggacaagac gcctatgtcc gagcgcctgg atgacaccga gccctacttc
     1561 attgggatct tctgcttcga ggcggggatc aagatcctgg ccctgggctt tgttctgcac
     1621 aagggctcct atctgcggaa cggctggaac gtcatggact tcgtggtagt cctcacgggg
     1681 attcttgcca cagccggaac cgacttcgac ctgcgaacgc tgagggctgt gcgtgtgcta
     1741 aggcccctga agctggtgtc tgggattccg agcttgcagg tggtgctcaa gtccatcatg
     1801 aaggccatgg tcccgctgct gcagatcggg ctgctgctct tcttcgccat cctcatgttt
     1861 gccatcatcg gcctcgagtt ctacatgggc aagttccaca aggcctgctt ccccaacagc
     1921 acagacccag atcccgtggg tgacttcccc tgtggcaagg aggccccggc ccggctctgt
     1981 gagggagaca cggagtgccg ggagtactgg gccggaccca actttggtat caccaacttt
     2041 gacaacatcc tgtttgccat cttgaccgtg ttccagtgca tcaccatgga gggctggact
     2101 gacatcctct acaatacaaa cgatgcagcc ggcaacacgt ggaactggct ctacttcatc
     2161 cctctcatca tcatcggctc cttcttcatg ctcaacctgg tgctgggtgt gctctcagga
     2221 gagtttgcta aggagcgcga gagagtggag aaccgccgcg ccttcctgaa gctccggcgg
     2281 cagcagcaga ttgagcggga gctcaacggg tacctggagg cgatcttcaa ggctgaggaa
     2341 gtcatgctgg ctgaggagga caggaacgca gaggagaagt cccctctgga tgcagtgttg
     2401 aagagagcag ctgccaagaa gagcagaagc gacctgatcc aggccgagga gggggagggc
     2461 cggctcacag gcctctgtgc tcccggttcc ccgttcgccc gtgccagcct gaaaagcggg
     2521 aagacggaga gctcgtcgta cttccggagg aaggagaaga tgttccgctt cttcatccgg
     2581 cgcatggtga aggcacagag cttctactgg acggtgctgt gtgtggtggc actgaacaca
     2641 ctgtgcgtgg ccatggtgca ctacaaccag ccgcagcggc tcaccacggc attgtacttt
     2701 gcagagtttg ttttcctggg tctcttcctc acagagatgt ccctgaagat gtatggcctg
     2761 gggcccagga gctacttccg gtcctccttc aactgttttg actttggggt catcgtgggc
     2821 agtatctttg aagtggtctg ggctgctgtc aagccaggga cctccttcgg gatcagtgtt
     2881 ctgcgggctc tgcgcttact gagaatcttc aaggtcacca aatactggaa ctccctacgg
     2941 aacctggtgg tgtctctgct caactccatg aagtccatca tcagcctcct cttcctgctc
     3001 ttcctgttca ttgttgtctt tgctctgctg gggatgcagc tgtttggagg ccagttcaac
     3061 tttaaagacg agaccccaac gaccaacttt gacaccttcc ctgctgctat cctcaccgtc
     3121 ttccagatcc tgaccggaga ggactggaat gcggtcatgt accacgggat cgagtcgcaa
     3181 ggtggcgtca gcagaggaat gttctcctcc ttctacttca ttgtcctcac tctgtttggg
     3241 aactacaccc tgctgaatgt cttcctggcc attgctgtgg acaaccttgc caatgctcag
     3301 gagctgacca aggatgaaga ggagatggaa gaagcagcca atcagaaact cgccttgcag
     3361 aaggccaagg aagtggctga agtcagcccc atgtctgctg cgaacatctc cattgccgcc
     3421 aggcagcaga actcggccaa agcgcgctcg gtgtgggagc agcgagccag ccagctgcgg
     3481 ctgcagaacc tgagggccag ctgcgaggcg ctgtacagcg agatggaccc cgaggagagg
     3541 ctgcgctacg ccaccgcgcg ccacctgcgg cccgacgtga agacgcacct ggaccggccg
     3601 ctggtggttg agcccggccg cgacgctccg cggggccccc cgggggggaa gtcccggccg
     3661 gatggctcgg aggcccccga gggtgcggat cccccgcgca ggcatcaccg gcaccgagac
     3721 aaggacaagg ctcccgccac ggtcccctcg gcgggggaac aggacagagc cgaagctctg
     3781 agggcagagg gcggggagct ggggccccgc gaggagcggg ggcggccacg ccgcagccgc
     3841 agcaaggagg ccccgggcgc cccggaggtg cgcagcgatc gcggccgtgg cccgtgtcct
     3901 gaaggtggtc ggcgccacca ccggcgcggg tcaccggagg aggcagctga gagggagccc
     3961 cgccgccacc gcgcgcaccg gcacgggccc gacccgggca aggagggccc agcgtccggg
     4021 accaggggcg agcggcgcgc acgacaccgc acgggccccc gggcgtgccc ccgggaggct
     4081 gagagcagcg aggagcctgc gcggcggcac cgggctcggc acaaggcgcc gcccacccag
     4141 gagaccgcgg agaaggacaa ggaggctgcc gagaaggggg gcgaggccac ggaggccgag
     4201 aaggacaagg aggctcggaa ccaccagccc aaggagctgc cctgtgacct ggaggccatc
     4261 ggcatgctgg gagtgggcgc tgtgcatacg ctgcccagca cctgtctgca gaaggtggag
     4321 gagcagccag aggatgcgga caaccagcgg aacgtcaccc gcatgggtag tcagcccccg
     4381 gacaccagca ccactgtgca cattcctgtg acgctgaccg gcccccccgg ggagaccacg
     4441 gtagtgccca gtggaaacgt ggacctggaa agtcaagcag aggggaagaa ggaggtggaa
     4501 accagcgatg tgatgaggag cggccctcgg cccattgtcc cgtacagttc catgttctgt
     4561 ttaagcccaa ccaacctgct gcgccgctgc tgccactaca ttgtgaccat gcgatacttc
     4621 gagatggtca tcctcgtggt catcgccctg agcagcatcg ccctggctgc cgaggacccc
     4681 gtgagaacag actctcccag gaataatgct ctgaagtaca tggactacat cttcacaggc
     4741 gtcttcacct tcgagatggt gataaagatg atcgatttgg gactgctgct acaccctgga
     4801 gcctatttcc gggatctgtg gaacattctg gacttcattg tggtcagtgg ggccctggtg
     4861 gcatttgctt tctcaggatc caaagggaaa gacatcagca ccatcaaatc cctgagagtc
     4921 ctgcgtgtgc tgcggccact caagaccatc aagcggctgc ccaagctcaa ggccgtgttt
     4981 gactgtgtgg tgaactcact gaaaaacgtc ctcaacatcc tgatcgtcta catgctcttc
     5041 atgttcatct ttgccgtcat tgccgtgcag ctcttcaaag ggaagttctt ctactgcacg
     5101 gacgaatcca aggagctgga gcgtgactgc aggggtcagt atttggatta tgagaaggag
     5161 gaagtggaag ctcaaccgag gcagtggaag aaatatgact tccactatga caacgtgctc
     5221 tgggctctgc taacgctctt cacggtgtcc acaggggaag ggtggcccat ggtgctgaaa
     5281 cactctgtgg atgccaccta cgaggagcag ggcccgagcc ccgggtaccg catggagctc
     5341 tccatcttct acgtggtcta ctttgtggtc tttcccttct tctttgtcaa catcttcgtg
     5401 gccttgatca tcatcacctt ccaggagcag ggggacaagg tgatgtccga gtgcagcctg
     5461 gagaagaacg agagggcttg catcgacttt gccatcagcg ccagacccct gacgcggtac
     5521 atgccccaga acaagcagtc gttccagtat aagacgtgga cgttcgtggt gtccccacct
     5581 tttgagtact tcatcatggc tatgatcgcc ctcaacactg tggtgctgat gatgaagttc
     5641 tacgatgcgc cgtacgagta tgagctaatg ctgaaatgcc tgaacatcgt gttcacgtcc
     5701 atgttctcca tggagtgtgt gctgaagatc atcgccttcg gggtgctgaa ctatttcaga
     5761 gatgcctgga atgtctttga ctttgtcacg gtgttgggaa gtattactga tattttggta
     5821 acagagattg cgaacaactt catcaacctc agcttcctgc gcctgttccg ggccgcgcgg
     5881 ctcatcaagc tgctgcgcca gggctacacc atccgcatcc tgctttggac cttcgtgcag
     5941 tccttcaagg cgctgcccta cgtgtgtctc ctcatcgcca tgctcttctt catctacgcc
     6001 atcattggca tgcaggtttt tgggaacatc gccctggacg atgacaccag catcaacagg
     6061 cacaataact tccggacgtt tctgcaggcc ttgatgttgc tgttcaggag cgccacgggg
     6121 gaggcctggc acgagataat gctgtcgtgc ctcagcagcc gggcctgcga cgagcactcc
     6181 aatgccagcg agtgcggcag cgactttgcc tacttttact tcgtctcctt catcttcctc
     6241 tgctcctttc tgatgttgaa cctctttgtg gctgtgatca tggacaattt tgagtacctt
     6301 acacgggact cttccatctt agggcctcac cacctggatg agttcatccg ggtctgggct
     6361 gaatacgacc cggccgcgtg tgggcgcatc agttacagtg acatgtttga gatgctgaag
     6421 cacatgtccc cacccctggg gctggggaag aagtgccctg ctcgagtcgc gtacaagcgc
     6481 ctggtccgca tgaacatgcc catctccagt gaggacatga cggtgcactt cacatccacg
     6541 ctgatggctc tcattcggac agcgctggac atcaagctgg caccagctgg gaccaagcag
     6601 caccagtgcg acgcagagct gaggaaggag atttcctgtg tgtgggccaa tctgccccag
     6661 aagaccctgg acttactggt accaccccat aagcctgacg agatgacggt gggcaaagtc
     6721 tatgcagctc tgatgatctt cgacttctac aaacagaaca aaaccagcag agaccagact
     6781 cagcaagccc ccggaggcct gtcccagctg ggccccgtgt ccctgttcca cccgctgaag
     6841 gccacgctgg agcagactca gccggcgctg cgtggagccc gggctttcct gcggcagaag
     6901 agttctgcct ccctgagcaa cggcggggcc gtacaaaccc aggagagcgg catcaaggaa
     6961 tcggtttctt ggggcactca gaggacccag gatgtgcttt gcgaggccag ggcacccctg
     7021 gagcggggcc actcagctga gatcccagtc gggcagcctg ggacactggc tgtagatgtc
     7081 cagatgcaga acatgactct gagtggccct gacgccgagc cgcagccagg gctggagagc
     7141 cagggccgag ccgcctccat gccccgcctg gccgcagaaa cacagcccgc gcctgacgcc
     7201 agccccatga agcgctccat ctccacgctg gccccgcgcc cccacacggc ccggctcggc
     7261 agcaccgcgc tggaccgccc agcccccagc caggccccgc accatcacca ccaccgctgc
     7321 caccgccgca gggacaggaa gcagcggtcc ctggagaagg gtcccagcct gtctgctgac
     7381 acagatgggg cccccgacag cacggtgggg ccaggactgc ccacggggga gggccccccc
     7441 ggctgcaggc gggagcggga gcgcaggcag gagcggggcc ggtcccagga gcggaggcag
     7501 ccctcgtcct cctcctcgga gaagcatcgc ttctactcct gtgaccgctt cgggggccgc
     7561 gaacccccac agcccaagcc ctccctcagt agtcacccca cgtcgccgac ggccgggcag
     7621 gagccgggac cccacccgca ggggagtggt tcggttcacg ggagccccct gctgtccaca
     7681 tctggtgcta gcaccccggg ccgggggcgg aggcagctcc cccagacccc cttgaccccc
     7741 cgccccagcg tcacctacaa gacggccaac tcctcgcctg tccacttcgc cggggcaccg
     7801 agcggcctcc ccgccttctc ccccggccgg ctcagccggg gcctctcaga acacaacgcc
     7861 ctgctgcaga gggacccgct cagccggccc ctggcgcccg gctcgcgcat cggctccgac
     7921 ccctacctgg ggcagcggct ggacagcgag gcgcccgccc gtgccctgcc cgaggacgcg
     7981 cccgcctttg aggagaccgc ggccagcaac tcgggccgct cctcccggac ctcgtacgtg
     8041 tcctcactga cctcgcagcc cccgccgctg cgccgcgtgc ccaacggcta ccactgcact
     8101 ctggggctgg gcgggggcgg ccgggcgcgg cgcggctgcc accaccccga ccgcgaccgc
     8161 cggtgctagc cgctgcgcgc ccgcccccgg cggtgggctt catcttccgc gctgggctgc
     8221 ggggacgcgg gcaccgaggc cctggacggg gcctgggcgg aggcccctgc tcccctcccc
     8281 ctcctccctg acagcagacg gatcaataaa gccccttccg gcgggacgcg gctgtctctg
     8341 ccccctgacg tgctgccttc ccaggtctcc caccacaggc tgatcctgtg tcagagaccc
     8401 cggtgtcagt gtgcaggggt ggggggtgcg gggcctgtgt cggaccccca gggttaatgg
     8461 ggccggaatt cctgcagccc ggggatccac tagttctagc aagcttcgac gaattccggg
     8521 gggggggggg ggggggacag ctcagggctg cgatttcgcg ccaaacttga cggcaatcct
     8581 agcgtgaagg ctggtaggat tttatccccg ctgccatcat ggttcgacca ttgaactgca
     8641 tcgtcgccgt gtcccaaaat atggggattg gcaagaacgg agacctaccc tggcctccgc
     8701 tcaggaacga gttcaagtac ttccaaagaa tgaccacaac ctcttcagtg gaaggtaaac
     8761 agaatctggt gattatgggt aggaaaacct ggttctccat tcctgagaag aatcgacctt
     8821 taaaggacag aattaatata gttctcagta gagaactcaa agaaccacca cgaggagctc
     8881 attttcttgc caaaagtttg gatgatgcct taagacttat tgaacaaccg gaattggcaa
     8941 gtaaagtaga catggtttgg atagtcggag gcagttctgt ttaccaggaa gccatgaatc
     9001 aaccaggcca cctcagactc tttgtgacaa ggatcatgca ggaatttgaa agtgacacgt
     9061 ttttcccaga aattgatttg gggaaatata aacttctccc agaataccca ggcgtcctct
     9121 ctgaggtcca ggaggaaaaa ggcatcaagt ataagtttga agtctacgag aagaaagact
     9181 aacaggaaga tgctttcaag ttctctgctc ccctcctaaa gctatgcatt ttttataaga
     9241 ccatgggact tttgctggct ttagatcata atcagccata ccacatttgt agaggtttta
     9301 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     9361 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9481 aatgtatctt atcatgtctg gatccccggc caacggtctg gtgacccggc tgcgagagct
     9541 cggtgtacct gagacgcgag taagcccttg agtcaaagac gtagtcgttg caagtccgca
     9601 ccaggtactg atatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta
     9661 gggtggccgg ggctccgggg gcgaggtctt ccaacataag gcgatgatat ccgtagatgt
     9721 acctggacat ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc
     9781 ggttccagat gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtga
     9841 ggcgtgcgca gtcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc
     9901 ttccgtggtc tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgaacc
     9961 ccggatccgg ccgtccgccg tgatccatcc ggttaccgcc cgcgtgtcga acccaggtgt
    10021 gcgacgtcag acaacggggg agcgctcctt ttggcttcct tccaggcgcg gcggctgctg
    10081 cgctagcttt tttggcgagc tcgaattaat tctgcattaa tgaatcggcc aacgcgcggg
    10141 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
    10561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    10801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11101 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11161 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11221 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11281 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11341 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11401 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11461 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11521 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12061 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    12121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    12181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    12241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    12301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    12361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    12421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    12481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    12541 acgttgtaaa acgacggcca gtgccaagct t
//
