LOCUS       sequence-90823-e       13228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of GFP tagged rabbit Cav2.2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin 
            AC
  TITLE     Dominant-negative synthesis suppression of voltage-gated calcium 
            channel Cav2.2 induced by truncated constructs.
  JOURNAL   J Neurosci. 2001 Nov 1;21(21):8495-504.
  PUBMED    11606638
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2001-11-1"; volume: "21(21)"; pages: "8495-504"
COMMENT     Sequence Label: Rabbit GFP Cav 2.2 pMT2
FEATURES             Location/Qualifiers
     source          1..13228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1104..1817
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1104..1817
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1134..1162)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1757..1777
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             9276..9839
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    10027..10161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10064..10083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10118..10137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10834..10851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11005..11593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11085..11104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11764..12555,12556..12624))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12387..12406
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12625..12729)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12797..12815
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12853..12875)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12975..12994
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13188..13210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13202..13219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13203..13219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attcctcgag
     1081 ggtaccgagc tcggatctaa aaaatgagta aaggagaaga acttttcact ggagttgtcc
     1141 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1201 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1261 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     1321 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     1381 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     1441 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     1501 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     1561 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     1621 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     1681 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     1741 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1801 tggatgaact atacaaaatg gtccgcttcg gggacgagct gggcggccgc tatgggggcg
     1861 cgggcggcgc ggagcgggcc cggggcggcg gggccggcgg ggctggcggc ccgggccccg
     1921 gggggctgcc gccgggccag cgggtcctct acaaacagtc gatcgcgcag cgcgcgcgga
     1981 ccatggcgct gtacaacccc atcccggtca agcagaactg cttcaccgtc aaccgctcgc
     2041 tcttcgtctt cagcgaggac aacgtcgtcc gcaaatacgc caagcgcatc accgagtggc
     2101 ctccgttcga gtacatgatc ctggccacca tcatcgccaa ctgcatcgtg ctggccctgg
     2161 agcagcacct ccctgacggg gacaagacgc ctatgtccga gcgcctggat gacaccgagc
     2221 cctacttcat tgggatcttc tgcttcgagg cggggatcaa gatcctggcc ctgggctttg
     2281 ttctgcacaa gggctcctat ctgcggaacg gctggaacgt catggacttc gtggtagtcc
     2341 tcacggggat tcttgccaca gccggaaccg acttcgacct gcgaacgctg agggctgtgc
     2401 gtgtgctaag gcccctgaag ctggtgtctg ggattccgag cttgcaggtg gtgctcaagt
     2461 ccatcatgaa ggccatggtc ccgctgctgc agatcgggct gctgctcttc ttcgccatcc
     2521 tcatgtttgc catcatcggc ctcgagttct acatgggcaa gttccacaag gcctgcttcc
     2581 ccaacagcac agacccagat cccgtgggtg acttcccctg tggcaaggag gccccggccc
     2641 ggctctgtga gggagacacg gagtgccggg agtactgggc cggacccaac tttggtatca
     2701 ccaactttga caacatcctg tttgccatct tgaccgtgtt ccagtgcatc accatggagg
     2761 gctggactga catcctctac aatacaaacg atgcagccgg caacacgtgg aactggctct
     2821 acttcatccc tctcatcatc atcggctcct tcttcatgct caacctggtg ctgggtgtgc
     2881 tctcaggaga gtttgctaag gagcgcgaga gagtggagaa ccgccgcgcc ttcctgaagc
     2941 tccggcggca gcagcagatt gagcgggagc tcaacgggta cctggagtgg atcttcaagg
     3001 ctgaggaagt catgctggct gaggaggaca ggaacgcaga ggagaagtcc cctctggatg
     3061 cagtgttgaa gagagcagct gccaagaaga gcagaagcga cctgatccag gccgaggagg
     3121 gggagggccg gctcacaggc ctctgtgctc ccggttcccc gttcgcccgt gccagcctga
     3181 aaagcgggaa gacggagagc tcgtcgtact tccggaggaa ggagaagatg ttccgcttct
     3241 tcatccggcg catggtgaag gcacagagct tctactggac ggtgctgtgt gtggtggcac
     3301 tgaacacact gtgcgtggcc atggtgcact acaaccagcc gcagcggctc accacggcat
     3361 tgtactttgc agagtttgtt ttcctgggtc tcttcctcac agagatgtcc ctgaagatgt
     3421 atggcctggg gcccaggagc tacttccggt cctccttcaa ctgttttgac tttggggtca
     3481 tcgtgggcag tatctttgaa gtggtctggg ctgctgtcaa gccagggacc tccttcggga
     3541 tcagtgttct gcgggctctg cgcttactga gaatcttcaa ggtcaccaaa tactggaact
     3601 ccctacggaa cctggtggtg tctctgctca actccatgaa gtccatcatc agcctcctct
     3661 tcctgctctt cctgttcatt gttgtctttg ctctgctggg gatgcagctg tttggaggcc
     3721 agttcaactt taaagacgag accccaacga ccaactttga caccttccct gctgctatcc
     3781 tcaccgtctt ccagatcctg accggagagg actggaatgc ggtcatgtac cacgggatcg
     3841 agtcgcaagg tggcgtcagc agaggaatgt tctcctcctt ctacttcatt gtcctcactc
     3901 tgtttgggaa ctacaccctg ctgaatgtct tcctggccat tgctgtggac aaccttgcca
     3961 atgctcagga gctgaccaag gatgaagagg agatggaaga agcagccaat cagaaactcg
     4021 ccttgcagaa ggccaaggaa gtggctgaag tcagccccat gtctgctgcg aacatctcca
     4081 ttgccgccag gcagcagaac tcggccaaag cgcgctcggt gtgggagcag cgagccagcc
     4141 agctgcggct gcagaacctg agggccagct gcgaggcgct gtacagcgag atggaccccg
     4201 aggagaggct gcgctacgcc accgcgcgcc acctgcggcc cgacgtgaag acgcacctgg
     4261 accggccgct ggtggttgag cccggccgcg acgctccgcg gggccccccg ggggggaagt
     4321 cccggccgga tggctcggag gcccccgagg gtgcggatcc cccgcgcagg catcaccggc
     4381 accgagacaa ggacaaggct cccgccacgg tcccctcggc gggggaacag gacagagccg
     4441 aagctctgag ggcagagggc ggggagctgg ggccccgcga ggagcggggg cggccacgcc
     4501 gcagccgcag caaggaggcc ccgggcgccc cggaggtgcg cagcgatcgc ggccgtggcc
     4561 cgtgtcctga aggtggtcgg cgccaccacc ggcgcgggtc accggaggag gcagctgaga
     4621 gggagccccg ccgccaccgc gcgcaccggc acgggcccga cccgggcaag gagggcccag
     4681 cgtccgggac caggggcgag cggcgcgcac gacaccgcac gggcccccgg gcgtgccccc
     4741 gggaggctga gagcagcgag gagcctgcgc ggcggcaccg ggctcggcac aaggcgccgc
     4801 ccacccagga gaccgcggag aaggacaagg aggctgccga gaaggggggc gaggccacgg
     4861 aggccgagaa ggacaaggag gctcggaacc accagcccaa ggagctgccc tgtgacctgg
     4921 aggccatcgg catgctggga gtgggcgctg tgcatacgct gcccagcacc tgtctgcaga
     4981 aggtggagga gcagccagag gatgcggaca accagcggaa cgtcacccgc atgggtagtc
     5041 agcccccgga caccagcacc actgtgcaca ttcctgtgac gctgaccggc ccccccgggg
     5101 agaccacggt agtgcccagt ggaaacgtgg acctggaaag tcaagcagag gggaagaagg
     5161 aggtggaaac cagcgatgtg atgaggagcg gccctcggcc cattgtcccg tacagttcca
     5221 tgttctgttt aagcccaacc aacctgctgc gccgctgctg ccactacatt gtgaccatgc
     5281 gatacttcga gatggtcatc ctcgtggtca tcgccctgag cagcatcgcc ctggctgccg
     5341 aggaccccgt gagaacagac tctcccagga ataatgctct gaagtacatg gactacatct
     5401 tcacaggcgt cttcaccttc gagatggtga taaagatgat cgatttggga ctgctgctac
     5461 accctggagc ctatttccgg gatctgtgga acattctgga cttcattgtg gtcagtgggg
     5521 ccctggtggc atttgctttc tcaggatcca aagggaaaga catcagcacc atcaaatccc
     5581 tgagagtcct gcgtgtgctg cggccactca agaccatcaa gcggctgccc aagctcaagg
     5641 ccgtgtttga ctgtgtggtg aactcactga aaaacgtcct caacatcctg atcgtctaca
     5701 tgctcttcat gttcatcttt gccgtcattg ccgtgcagct cttcaaaggg aagttcttct
     5761 actgcacgga cgaatccaag gagctggagc gtgactgcag gggtcagtat ttggattatg
     5821 agaaggagga agtggaagct caaccgaggc agtggaagaa atatgacttc cactatgaca
     5881 acgtgctctg ggctctgcta acgctcttca cggtgtccac aggggaaggg tggcccatgg
     5941 tgctgaaaca ctctgtggat gccacctacg aggagcaggg cccgagcccc gggtaccgca
     6001 tggagctctc catcttctac gtggtctact ttgtggtctt tcccttcttc tttgtcaaca
     6061 tcttcgtggc cttgatcatc atcaccttcc aggagcaggg ggacaaggtg atgtccgagt
     6121 gcagcctgga gaagaacgag agggcttgca tcgactttgc catcagcgcc agacccctga
     6181 cgcggtacat gccccagaac aagcagtcgt tccagtataa gacgtggacg ttcgtggtgt
     6241 ccccaccttt tgagtacttc atcatggcta tgatcgccct caacactgtg gtgctgatga
     6301 tgaagttcta cgatgcgccg tacgagtatg agctaatgct gaaatgcctg aacatcgtgt
     6361 tcacgtccat gttctccatg gagtgtgtgc tgaagatcat cgccttcggg gtgctgaact
     6421 atttcagaga tgcctggaat gtctttgact ttgtcacggt gttgggaagt attactgata
     6481 ttttggtaac agagattgcg aacaacttca tcaacctcag cttcctgcgc ctgttccggg
     6541 ccgcgcggct catcaagctg ctgcgccagg gctacaccat ccgcatcctg ctttggacct
     6601 tcgtgcagtc cttcaaggcg ctgccctacg tgtgtctcct catcgccatg ctcttcttca
     6661 tctacgccat cattggcatg caggtttttg ggaacatcgc cctggacgat gacaccagca
     6721 tcaacaggca caataacttc cggacgtttc tgcaggcctt gatgttgctg ttcaggagcg
     6781 ccacggggga ggcctggcac gagataatgc tgtcgtgcct cagcagccgg gcctgcgacg
     6841 agcactccaa tgccagcgag tgcggcagcg actttgccta cttttacttc gtctccttca
     6901 tcttcctctg ctcctttctg atgttgaacc tctttgtggc tgtgatcatg gacaattttg
     6961 agtaccttac acgggactct tccatcttag ggcctcacca cctggatgag ttcatccggg
     7021 tctgggctga atacgacccg gccgcgtgtg ggcgcatcag ttacagtgac atgtttgaga
     7081 tgctgaagca catgtcccca cccctggggc tggggaagaa gtgccctgct cgagtcgcgt
     7141 acaagcgcct ggtccgcatg aacatgccca tctccagtga ggacatgacg gtgcacttca
     7201 catccacgct gatggctctc attcggacag cgctggacat caagctggca ccagctggga
     7261 ccaagcagca ccagtgcgac gcagagctga ggaaggagat ttcctgtgtg tgggccaatc
     7321 tgccccagaa gaccctggac ttactggtac caccccataa gcctgacgag atgacggtgg
     7381 gcaaagtcta tgcagctctg atgatcttcg acttctacaa acagaacaaa accagcagag
     7441 accagactca gcaagccccc ggaggcctgt cccagctggg ccccgtgtcc ctgttccacc
     7501 cgctgaaggc cacgctggag cagactcagc cggcgctgcg tggagcccgg gctttcctgc
     7561 ggcagaagag ttctgcctcc ctgagcaacg gcggggccgt acaaacccag gagagcggca
     7621 tcaaggaatc ggtttcttgg ggcactcaga ggacccagga tgtgctttgc gaggccaggg
     7681 cacccctgga gcggggccac tcagctgaga tcccagtcgg gcagcctggg acactggctg
     7741 tagatgtcca gatgcagaac atgactctga gtggccctga cgccgagccg cagccagggc
     7801 tggagagcca gggccgagcc gcctccatgc cccgcctggc cgcagaaaca cagcccgcgc
     7861 ctgacgccag ccccatgaag cgctccatct ccacgctggc cccgcgcccc cacacggccc
     7921 ggctcggcag caccgcgctg gaccgcccag cccccagcca ggccccgcac catcaccacc
     7981 accgctgcca ccgccgcagg gacaggaagc agcggtccct ggagaagggt cccagcctgt
     8041 ctgctgacac agatggggcc cccgacagca cggtggggcc aggactgccc acgggggagg
     8101 gcccccccgg ctgcaggcgg gagcgggagc gcaggcagga gcggggccgg tcccaggagc
     8161 ggaggcagcc ctcgtcctcc tcctcggaga agcatcgctt ctactcctgt gaccgcttcg
     8221 ggggccgcga acccccacag cccaagccct ccctcagtag tcaccccacg tcgccgacgg
     8281 ccgggcagga gccgggaccc cacccgcagg ggagtggttc ggttcacggg agccccctgc
     8341 tgtccacatc tggtgctagc accccgggcc gggggcggag gcagctcccc cagaccccct
     8401 tgaccccccg ccccagcgtc acctacaaga cggccaactc ctcgcctgtc cacttcgccg
     8461 gggcaccgag cggcctcccc gccttctccc ccggccggct cagccggggc ctctcagaac
     8521 acaacgccct gctgcagagg gacccgctca gccggcccct ggcgcccggc tcgcgcatcg
     8581 gctccgaccc ctacctgggg cagcggctgg acagcgaggc gcccgcccgt gccctgcccg
     8641 aggacgcgcc cgcctttgag gagaccgcgg ccagcaactc gggccgctcc tcccggacct
     8701 cgtacgtgtc ctcactgacc tcgcagcccc cgccgctgcg ccgcgtgccc aacggctacc
     8761 actgcactct ggggctgggc gggggcggcc gggcgcggcg cggctgccac caccccgacc
     8821 gcgaccgccg gtgctagccg ctgcgcgccc gcccccggcg gtgggcttca tcttccgcgc
     8881 tgggctgcgg ggacgcgggc accgaggccc tggacggggc ctgggcggag gcccctgctc
     8941 ccctccccct cctccctgac agcagacgga tcaataaagc cccttccggc gggacgcggc
     9001 tgtctctgcc ccctgacgtg ctgccttccc aggtctccca ccacaggctg atcctgtgtc
     9061 agagaccccg gtgtcagtgt gcaggggtgg ggggtgcggg gcctgtgtcg gacccccagg
     9121 gttaatgggg ccggaattcc tcgaggtacc cgggactagt gcggccgcaa ttccgggggg
     9181 gggggggggg gggacagctc agggctgcga tttcgcgcca aacttgacgg caatcctagc
     9241 gtgaaggctg gtaggatttt atccccgctg ccatcatggt tcgaccattg aactgcatcg
     9301 tcgccgtgtc ccaaaatatg gggattggca agaacggaga cctaccctgg cctccgctca
     9361 ggaacgagtt caagtacttc caaagaatga ccacaacctc ttcagtggaa ggtaaacaga
     9421 atctggtgat tatgggtagg aaaacctggt tctccattcc tgagaagaat cgacctttaa
     9481 aggacagaat taatatagtt ctcagtagag aactcaaaga accaccacga ggagctcatt
     9541 ttcttgccaa aagtttggat gatgccttaa gacttattga acaaccggaa ttggcaagta
     9601 aagtagacat ggtttggata gtcggaggca gttctgttta ccaggaagcc atgaatcaac
     9661 caggccacct cagactcttt gtgacaagga tcatgcagga atttgaaagt gacacgtttt
     9721 tcccagaaat tgatttgggg aaatataaac ttctcccaga atacccaggc gtcctctctg
     9781 aggtccagga ggaaaaaggc atcaagtata agtttgaagt ctacgagaag aaagactaac
     9841 aggaagatgc tttcaagttc tctgctcccc tcctaaagct atgcattttt tataagacca
     9901 tgggactttt gctggcttta gatcataatc agccatacca catttgtaga ggttttactt
     9961 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10021 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10141 gtatcttatc atgtctggat ccccggccaa cggtctggtg acccggctgc gagagctcgg
    10201 tgtacctgag acgcgagtaa gcccttgagt caaagacgta gtcgttgcaa gtccgcacca
    10261 ggtactgata tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc cagcgtaggg
    10321 tggccggggc tccgggggcg aggtcttcca acataaggcg atgatatccg tagatgtacc
    10381 tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg cggacgcggt
    10441 tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg ccggtgaggc
    10501 gtgcgcagtc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg ggcactcttc
    10561 cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt tcgaaccccg
    10621 gatccggccg tccgccgtga tccatccggt taccgcccgc gtgtcgaacc caggtgtgcg
    10681 acgtcagaca acgggggagc gctccttttg gcttccttcc aggcgcggcg gctgctgcgc
    10741 tagctttttt ggcgagctcg aattaattct gcattaatga atcggccaac gcgcggggag
    10801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
    11221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    11461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    12781 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    12841 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    12901 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    12961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    13021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    13081 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    13141 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    13201 ttgtaaaacg acggccagtg ccaagctt
//
