LOCUS       sequence-90834-e         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha R E-F
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    107..131
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(217..522)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(319..338)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cctttttacg gttcctgtgg ttggcacata caaatggacg aacggataaa ccttttcacg
       61 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
      121 atatcctgtc aaacactgat agtttaaacc agaggtctca ctcatacgaa gtcttcgtcg
      181 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
      241 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
      301 ggaaaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
      361 caccaccggg taaagttcac gggggacttt atctgacagc agacgtgcac tggccagggg
      421 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
      481 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
      541 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctca
//
