LOCUS       sequence-90842-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 4-5
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..108
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    308..432
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(473..778)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(575..594)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcattaggc
       61 accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga agcggagagc
      121 gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga caggtttccc
      181 gactggaaag cgggcagtga gcggaaggcc catgaggcca gtcttgtgct ccattaatta
      241 agaggtacct agaattctgg gatccaagcg gccgctaccc cgaagacaac tcagacatga
      301 gaccccaacc actttgtaca agaaagctga acgagaaacg taaaatgata taaatatcaa
      361 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      421 ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc
      481 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
      541 cttcttcccc gataacggaa accggcacac tggccatatc ggtggtcatc atgcgccagc
      601 tttcatcccc gatatgcacc accgggtaaa gttcacgggg gactttatct gacagcagac
      661 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
      721 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
      781 caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca
      841 gccatcc
//
