LOCUS       sequence-90844-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 1-2
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    308..432
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(469..474,475..492,493..499)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(619..638)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcatta
       61 ggcaccccag gctttacccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggag
      121 agcgcccaat acgcaaggaa acagctatga ccatgttaat gcagctggca cgacaggttt
      181 cccgactgga aagcgggcag tgagcggaag gcccatgagg ccagtcttgt gctccattaa
      241 ttaagaggta cctagaattc tgggatccaa gcggccgcta ccccgaagac aatacggcgg
      301 tgagaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
      361 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      421 ccagtcacta tggcggcccc tcgaggggcc gcattaggca ccccaggctt tacactttat
      481 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      541 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      601 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      661 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      721 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      781 atgaaagacg gtgagctggt gatatgggat agtgt
//
