LOCUS       sequence-90848-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for human TRIM65 fusion protein with 
            c-terminal EGFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME
  TITLE     TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 
            10.1073/pnas.1322545111. Epub 2014 Apr 28.
  PUBMED    24778252
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1322545111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-13- 13"; volume: "111"; issue: "19"; pages: "6970-5"
COMMENT     Sequence Label: hTRIM65_EGFP_N1
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg ttcgactgca
       61 gaattcggct gagcacctct tcctggggcc ccggagggaa cacagcccct ggctgatggc
      121 acagggtcag ggtcctaccc tcgaggagcc agaagacggg ggtgaggggc tggttgaaga
      181 gggcatggaa ggtgtacagg ggctgggtct ggggctccag gctgtagaag gtgaggcagc
      241 ctgaggccag gtccaaatcc atgcccagga gccgccctga cacccctggg aggcgctggg
      301 cttccccgtt gtgccaggcc tggaggctgt cctcctggac gcagagcccc caggagcagg
      361 gtccccggcc aatgttgtct gtgtggggcc ccagcctgca ccgtggcagt tgcgggtagg
      421 agacgcccag tgtcaccgag tggtctgacg cgcgcacctc ccagtagtgg tgcccggcct
      481 ggaagctctg ggcacattgc acctgccaga gctcaaagct gccgggcccg cctgggcccc
      541 gggactgacg acagtgcttc acctgctggt cctggcgcga cagatagaag tgacggttgg
      601 cgctgactgg atcaaaggtc agattgcgat aattctgcca gagtttcctc ctcagtggac
      661 aaactgtgct tgggaccggt gccaggggac ctggggcctc cacgggggct aagtccacag
      721 gcttggctgg tgccccaggg tggctcccct cttccaagag gaggccacac agccggctta
      781 gcaactgctt caggtcaccc agctgttggt cttcatc
//
