LOCUS       sequence-90894-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express FLAG-tagged human UbxD8 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Xu Y, Liu Y, Lee JG, Ye Y
  TITLE     A ubiquitin-like domain recruits an oligomeric chaperone to a 
            retrotranslocation complex in endoplasmic reticulum-associated 
            degradation.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18068-76. doi: 
            10.1074/jbc.M112.449199. Epub 2013 May 12.
  PUBMED    23665563
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.449199"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18068-76"
COMMENT     Sequence Label: pRK-FLAG-UbxD8
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        225..243
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     225..242
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     256..275
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      315..324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             324..347
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
      121 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa
      181 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      241 agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc
      301 tcggttctaa gcttgccgcc atggactaca aggacgacga tgacaagggg tcgaccatgg
      361 cggcgcctga ggagcgggat ctaacccagg agcagacaga gaagctgctg cagtttcagg
      421 atctcactgg catcgaatct atggatcagt gtcgccatac cttggaacag cataactgga
      481 acatagaggc tgctgtacag gacagattga atgagcaaga gggcgtacct agtgttttca
      541 acccacctcc atcacgaccc ctgcaggtta atacagctga ccacaggatc tacagctatg
      601 ttgtctcaag acctcaacca agggggctgc ttggatgggg ttattacttg ataatgcttc
      661 cattccggtt tacctattac acgatacttg atatatttag gtttgctctt cgttttatac
      721 ggcctgaccc tcgcagccgg gtcactgacc ccgttgggga cattgtttca tttatgcact
      781 cttttgaaga gaaatatggg agggcacacc ctgtcttcta ccagggaacg tacagccagg
      841 cacttaacga tgccaaaagg gagct
//
