LOCUS       sequence-90919-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Wang HR, Zhang Y, Ozdamar B, Ogunjimi AA, Alexandrova E, Thomsen GH,
            Wrana JL
  TITLE     Regulation of cell polarity and protrusion formation by targeting 
            RhoA for degradation.
  JOURNAL   Science. 2003 Dec 5. 302(5651):1775-9.
  PUBMED    14657501
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2003 Dec 5. 302(5651):1775-9."
COMMENT     Sequence Label: pcDNA3-PKCZeta KR HA Tagged
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcctcgag gccaccatgg aattccccag caggaccgac
      121 cccaagatgg accggagcgg cggccgcgtc cgtctgaagg cgcactacgg cggggacatc
      181 ctgattacca gcgtggaccc cacgacaact ttccaggacc tctgtgagga agtgcgagac
      241 atgtgtggcc tgcaccagca gcacccactc accctcaagt gggtggacag tgaaggtgac
      301 ccttgtactg tgtcctcaca gatggagctg gaggaggcct tccgcctggc ctgtcagggc
      361 agggacgaag tgctcatcat ccacgttttc ccaagcatcc cagaacaacc gggcatgcct
      421 tgtcctggag aagacaagtc catctaccgc cgtggagcca gaagatggag gaagctatac
      481 cgagccaacg gccacctctt ccaagccaag cgctttaaca ggagagcgta ctgtggccag
      541 tgcagcgaaa ggatatgggg cctcgcgagg caggggtaca ggtgcatcaa ctgcaagctg
      601 cttgtccata aacgctgcca cgtcctcgtc ccgctgacct gcaggaggca tatggattct
      661 gtcatgcctt cccaagagcc tccagtagat gacaagaacg atggtgtaga ccttccttca
      721 gaagaaactg atggaattgc ttatatttct tcatctcgga aacatgacaa tatcaaagat
      781 gattctgagg accttaagcc tgtcatcgat ggggtggatg ggatcaaaat ctctcagggg
      841 ctggggctgc aagactttga cctcatcaga gtcatcggg
//
