LOCUS       sequence-90956-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mApple-C1
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..747
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     453..472
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
       61 aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      121 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga ggcctttcag
      181 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      241 cctcagttca tgtacggctc caaggtctac attaagcacc cagccgacat ccccgactac
      301 ttcaagctgt ccttccccga gggcttcagg tgggagcgcg tgatgaactt cgaggacggc
      361 ggcattattc acgttaacca ggactcctcc ctgcaggacg gcgtgttcat ctacaaggtg
      421 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
      481 tgggaggcct ccgaggagcg gatgtacccc gaggacggcg ccctgaagag cgagatcaag
      541 aagaggctga agctgaagga cggcggccac tacgccgccg aggtcaagac cacctacaag
      601 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcgtg
      661 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
      721 accggcggca tggacgagct gtacaagt
//
