LOCUS       sequence-90992-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated 
            transformation). There is a 69-bp MCS (multiple cloning site) which 
            contains 12 unique restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Yang F, Naqvi NI
  TITLE     Sulfonylurea resistance reconstitution as a novel strategy for 
            ILV2-specific integration in Magnaporthe oryzae.
  JOURNAL   Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005.
  PUBMED    24769367
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005."
COMMENT     Sequence Label: pFGL815
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    49..223
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(252..268)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(252..268)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(296..313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(296..312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(305..327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    515..539
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 attggcggac gtttttatgt actgaattaa cgccgaatta attcggggga tctggatttt
       61 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
      121 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct
      181 aattccctta tctgggaact actcacacat tattatggag aaactcgaga attcgagctc
      241 ggtacccggg ggatccacta gttctagagt cgacctgcag gcatgcaagc ttggcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
      481 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
      541 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
      601 ccgttggtcc atttgtatgt ccatgccaac cacagggttc ccctcgggat caaagtactt
      661 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
      721 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
      781 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
      841 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
      901 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
      961 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     1021 ttgaccacct acgc
//
