LOCUS       sequence-91-expo        7233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_07
FEATURES             Location/Qualifiers
     source          1..7233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             150..170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(260..282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4673..4695)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4795..4814
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4892..4910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4978..5082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5083..5151,5152..5943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5301..5320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6114..6702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6603..6622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6856..6873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6990..7011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7026..7031,7032..7049,7050..7056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7064..7080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7069..7091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7088..7104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7088..7104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga
      121 gggtaccgag ctcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtgggtg
      181 aagaccagaa acagcacctc gaactgagcc gcgatattgc ccagcgtttc aacgcgctgt
      241 atggcgagat cgatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      301 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      361 gcaccgatcg cccttcccaa cagttgcgaa ggtaagttta aacagatcca tactaactaa
      421 cttgttctga cataattttc agcttgaatg gcgaatggcg ctttgcctgg tttccggcac
      481 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
      541 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
      601 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
      661 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
      721 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggtaagtt
      781 taattaagtt gatactaact aacaagatct gattaatttt caggacagtc gtttgccgtc
      841 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
      901 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
      961 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
     1021 tcgctttaat gatgatttct cccgcgctgt actggaggct gaagtccagg taagtttaaa
     1081 caggatctta ctaactaaca tgctaacact gaattttcag atgtgcggcg agttgcgtga
     1141 ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
     1201 gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
     1261 tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
     1321 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
     1381 ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
     1441 aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
     1501 ggtgcaggat gtaagtttaa actattcgtt actaactaac tttaaacatt taaattttca
     1561 gatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
     1621 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
     1681 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
     1741 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
     1801 ctggtcgctg gggaatggta agtttaaaca gttgaatact aactaacgga gatctttgaa
     1861 attttcagaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt
     1921 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat
     1981 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg
     2041 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgattc tttgcgaggt
     2101 aagtttaaac agaactctac taactaacac attagatcct aattttcagt acgctcacgc
     2161 gatgggcaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     2221 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     2281 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     2341 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgtaag tttaaacaat
     2401 aacctactaa ctaacgtaga taatttaaat tttcaggctg acggaagcaa aacaccagca
     2461 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     2521 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     2581 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     2641 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     2701 cgcgaccgca tggtcagaag ccgggcacat cagcgcatgg cagcagtgga ggtaagttta
     2761 aacaagatcc tactaactaa ctctacattg atgaattttc agactggcgg aaaacctcag
     2821 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt
     2881 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca
     2941 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
     3001 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt
     3061 cggtaagttt aaacaaagtt gtactaacta acgaagatct tgataatttt cagaacgctg
     3121 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
     3181 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
     3241 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
     3301 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
     3361 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
     3421 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtagt aagtttaaac
     3481 ttgatagtac taactaacat gtttcattta aattttcagt accccgtacg tcttcccgag
     3541 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
     3601 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
     3661 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
     3721 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccaactga gcgccggtcg
     3781 ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt
     3841 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg
     3901 cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     3961 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
     4021 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     4081 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     4141 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat
     4201 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca
     4261 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa
     4321 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct
     4381 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct
     4441 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
     4501 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg
     4561 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttcctacta
     4621 gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4681 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4741 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     4801 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     4861 ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt
     4921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6241 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6481 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     6541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6781 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6841 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6901 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6961 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7021 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7081 tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct
     7141 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
     7201 acgatggata cgctaacaac ttggaaatga aat
//
