LOCUS       sequence-91006-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter assay for CIP2A with point mutation on miR-375 
            binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang
            LJ, Yang LJ, Chan EK
  TITLE     Tumor suppressor miR-375 regulates MYC expression via repression of 
            CIP2A coding sequence through multiple miRNA-mRNA interactions.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 
            10.1091/mbc.E12-12-0891. Epub 2013 Apr 3.
  PUBMED    23552692
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-12-0891"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "11"; pages: "1638-48, S1-7"
COMMENT     Sequence Label: pMiR-CIP2A-miR-375-mutC&D
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..793
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             805..834
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             853..876
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(896..912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(896..912)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgtttagtga accgtcagaa ttttgtaata cgactcacta tagggcggcc gggaatcgtc
       61 gactggatcc agtaccgagg agatctgcgc cgcgttcgcc atgagcgagc tgatcaagga
      121 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
      181 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgaagatca aggtggtcga
      241 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
      301 agccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg
      361 cttcacatgg gagagaatca ccacatacga agacgggggc gtgctgaccg ctacccagga
      421 caccagcttc cagaacggct gcatcatcta caacgtcaag atcaacgggg tgaacttccc
      481 atccaacggc cctgtgatgc agaagaaaac acgcggctgg gaggccaaca ccgagatgct
      541 gtaccccgct gacggcggcc tgagaggcca cagccagatg gccctgaagc tcgtgggcgg
      601 gggctacctg cactgctcct tcaagaccac atacagatcc aagaaacccg ctaagaacct
      661 caagatgccc ggcttccact tcgtggacca cagactggaa agaatcaagg aggccgacaa
      721 agagacctac gtcgagcagc acgagatggc tgtggccaag tactgcgacc tccctagcaa
      781 actggggcac agatcgcgtc cctcgagcag aaactcatct cagaagagga tctggcagca
      841 aatgatatcc tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat
      901 agctgtttcc tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct
      961 ggccctggaa gttgccactc cagtgcc
//
