LOCUS       sequence-91047-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCherry-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..813
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(251..269)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     519..538
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc
      121 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      181 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      241 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      301 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      361 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      541 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      601 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      661 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      781 cactccaccg gcggcatgga cgagctgtac aagtccggat cca
//
