LOCUS       sequence-91052-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: YPet-C1
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(39..41,42..44,45..755)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(345..364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaaa ggcgaagagc
       61 tgttcaccgg cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt
      121 tcagcgtgag cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagctgc
      181 tgtgcaccac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ctgggctacg
      241 gcgtgcagtg cttcgcccgg taccccgacc acatgaagca gcacgacttc ttcaagagcg
      301 ccatgcccga gggctacgtg caggagcgga ccatcttctt caaggacgac ggcaactaca
      361 agacccgggc cgaggtgaag ttcgagggcg acaccctggt gaaccggatc gagctgaagg
      421 gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
      481 gccacaacgt gtacatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
      541 tccggcacaa catcgaggac ggcggcgtgc agctggccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagagcgccc
      661 tgttcaagga ccccaacgag aagcgggacc acatggtgct gctggagttc ctgaccgccg
      721 ccggcatcac cgagggcatg aacgagctct ataagtccgg actcagatct cgagctcaag
      781 cttcgaattc tgcagtcgac ggtaccgcgg gcccg
//
