LOCUS       sequence-91054-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. 
            This plasmid encodes dClover2 A206K, H231L based on publication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: dClover2-C1
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..755
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(84..105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     692..713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
       61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      121 tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
      181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
      241 gcgtggcctg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      301 ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca
      361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
      481 gccactacgt ctatatcacg gccgacaagc agaagaacag catcaaggct aacttcaaga
      541 tccgccacaa cgttgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccaagc
      661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      721 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
      781 cttcgaattc tgcagtc
//
