LOCUS       sequence-91095-e       20831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses recombinant measles virus with duplicated P gene. wt P 
            gene and P-F497D gene are tagged with Flag and HA peptides and si1 
            (siGFP) and si2 (siP) target sequences, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20831)
  TITLE     biG-biS plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p(+)MV-NSE-FlagrP-3g_haP-F497D-3p
FEATURES             Location/Qualifiers
     source          1..20831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1213..1221
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1810..1833
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2853..2864
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3592..3618
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4641..4652
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             11826..11837
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17924..17942)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      17938..17985
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(18038..18056)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(18077..18093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18077..18093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(18090..18112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    18101..18117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18125..18131,18132..18149,18150..18155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18170..18191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(18308..18325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18479..19067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18559..18578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19238..20029,20030..20098))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19861..19880
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20099..20203)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(20366..20387)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     20777..20799
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     20791..20808
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20792..20808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accaaacaaa gttgggtaag gatagttcaa tcaatgatca tcttctagtg cacttaggat
       61 tcaagatcct attatcaggg acaagagcag gattagggat atccgagatg gccacacttt
      121 taaggagctt agcattgttc aaaagaaaca aggacaaacc acccattaca tcaggatccg
      181 gtggagccat cagaggaatc aaacacatta ttatagtacc aatccctgga gattcctcaa
      241 ttaccactcg atccagactt ctggaccggt tggtcaggtt aattggaaac ccggatgtga
      301 gcgggcccaa actaacaggg gcactaatag gtatattatc cttatttgtg gagtctccag
      361 gtcaattgat tcagaggatc accgatgacc ctgacgttag cataaggctg ttagaggttg
      421 tccagagtga ccagtcacaa tctggcctta ccttcgcatc aagaggtacc aacatggagg
      481 atgaggcgga ccaatacttt tcacatgatg atccaattag tagtgatcaa tccaggttcg
      541 gatggttcga gaacaaggaa atctcagata ttgaagtgca agaccctgag ggattcaaca
      601 tgattctggg taccatccta gcccaaattt gggtcttgct cgcaaaggcg gttacggccc
      661 cagacacggc agctgattcg gagctaagaa ggtggataaa gtacacccaa caaagaaggg
      721 tagttggtga atttagattg gagagaaaat ggttggatgt ggtgaggaac aggattgccg
      781 aggacctctc cttacgccga ttcatggtcg ctctaatcct ggatatcaag agaacacccg
      841 gaaacaaacc caggattgct gaaatgatat gtgacattga tacatatatc gtagaggcag
      901 gattagccag ttttatcctg actattaagt ttgggataga aactatgtat cctgctcttg
      961 gactgcatga atttgctggt gagttatcca cacttgagtc cttgatgaac ctttaccagc
     1021 aaatggggga aactgcaccc tacatggtaa tcctggagaa ctcaattcag aacaagttca
     1081 gtgcaggatc ataccctctg ctctggagct atgccatggg agtaggagtg gaacttgaaa
     1141 actccatggg aggtttgaac tttggccgat cttactttga tccagcatat tttagattag
     1201 ggcaagagat ggtaaggagg tcagctggaa aggtcagttc cacattggca tctgaactcg
     1261 gtatcactgc cgaggatgca aggcttgttt cagagattgc aatgcatact actgaggaca
     1321 agatcagtag agcggttgga cccagacaag cccaagtatc atttctacac ggtgatcaaa
     1381 gtgagaatga gctaccgaga ttggggggca aggaagatag gagggtcaaa cagagtcgag
     1441 gagaagccag ggagagctac agagaaaccg ggcccagcag agcaagtgat gcgagagctg
     1501 cccatcttcc aaccggcaca cccctagaca ttgacactgc atcggagtcc agccaagatc
     1561 cgcaggacag tcgaaggtca gctgacgccc tgcttaggct gcaagccatg gcaggaatct
     1621 cggaagaaca aggctcagac acggacaccc ctatagtgta caatgacaga aatcttctag
     1681 actaggtgcg agaggccgag gaccagaaca acatccgcct accctccatc attgttataa
     1741 aaaacttagg aaccaggtcc acacagccgc cagcccatca accatccact cccacgattg
     1801 gagcagatgg attataagga tgacgatgac aaagcagaag agcaggcacg ccatgtcaaa
     1861 aacggactgg aatgcatccg ggctctcaag gccgagccca tcggctcact ggccatcgag
     1921 gaagctatgg cagcatggtc agaaatatca gacaacccag gacaggagcg agccacctgc
     1981 agggaagaga aggcaggcag ttcgggtctc agcaaaccat gcctctcagc aattggatca
     2041 actgaaggcg gtgcacctcg catccgcggt cagggacctg gagagagcga tgacgacgct
     2101 gaaactttgg gaatcccccc aagaaatctc caggcatcaa gcactgggct acagtgtcat
     2161 tatgtttatg atcacagcgg tgaagcggtt aagggaatcc aagatgctga ctctatcatg
     2221 gttcaatcag gccttgatgg tgatagcacc ctctcaggag gagacaatga atctgaaaac
     2281 agcgatgtgg atattggcga acctgatacc gagggatatg ctatcactga ccggggatct
     2341 gctcccatct ctatggggtt cagggcttct gatgttgaaa ctgcagaagg aggggagatc
     2401 cacgagctcc tgagactcca atccagaggc aacaactttc cgaagcttgg gaaaactctc
     2461 aatgttcctc cgcccccgga ccccggtagg gccagcactt ccgggacacc cattaaaaag
     2521 ggcacagacg cgagattagc ctcatttgga acggagatcg cgtcttcatt gacaggtggt
     2581 gcaacccaat gtgctcgaaa gtcaccctcg gaaccatcag ggccaggtgc acctgcgggg
     2641 aacgtccccg agtgtgtgag caatgccgca ctgatacagg agtggacacc cgaatctggc
     2701 accacaatct ccccgagatc ccagaataat gaagaagggg gagaccatta tgatgatgag
     2761 ctgttctctg atgtccaaga tattaaaaca gccttggcca aaatacacga ggataatcag
     2821 aagataatct ccaagctaga atcactgctg ttattgaagg gagaagttga gtcaattaag
     2881 aagcagatca acaggcaaaa tatcagcata tccaccctgg aaggccacct tagctcaatc
     2941 atgatcgcca ttcctggact tgggaaggat cccaacgacc ccactgcaga tgtcgaaatc
     3001 aatcccgact tgaaacccat cataggcaga gattcaggcc gagcactggc cgaagttctc
     3061 aagaaacccg ttgccagccg acaactccaa ggaatgacaa atggacggac cagttccaga
     3121 ggacagctgc tgaaggaatt tcagctaaag ccgatcggga aaaagatgag ctcagccgtc
     3181 gggtttgttc ctgacaccgg ccctgcatca cgcagtgtaa tccgctccat tataaaatcc
     3241 agccggctag aggaggatcg gaagcgttac ctgatgactc tccttgatga tatcaaagga
     3301 gccaatgatc ttgccaagtt ccaccagatg ctggtgaaga taataatgaa gtagctacag
     3361 ctcaacttac ctgccaaccg aacggcatca aggtgaactc catgcgaacg gcatcaaggt
     3421 gaactcagtc ggaacggcat caaggtgaac tacccaacta gcgtacggtg cgagaggccg
     3481 aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta ggaaccaggt
     3541 ccacacagcc gccagcccat caaccatcca ctcccacgat tggagcagat gtacccatac
     3601 gatgttccag attacgctgg agcagaagag caggcacgcc atgtcaaaaa cggactggaa
     3661 tgcatccggg ctctcaaggc cgagcccatc ggctcactgg ccatcgagga agctatggca
     3721 gcatggtcag aaatatcaga caacccagga caggagcgag ccacctgcag ggaagagaag
     3781 gcaggcagtt cgggtctcag caaaccatgc ctctcagcaa ttggatcaac tgaaggcggt
     3841 gcacctcgca tccgcggtca gggacctgga gagagcgatg acgacgctga aactttggga
     3901 atccccccaa gaaatctcca ggcatcaagc actgggctac agtgtcatta tgtttatgat
     3961 cacagcggtg aagcggttaa gggaatccaa gatgctgact ctatcatggt tcaatcaggc
     4021 cttgatggtg atagcaccct ctcaggagga gacaatgaat ctgaaaacag cgatgtggat
     4081 attggcgaac ctgataccga gggatatgct atcactgacc ggggatctgc tcccatctct
     4141 atggggttca gggcttctga tgttgaaact gcagaaggag gggagatcca cgagctcctg
     4201 agactccaat ccagaggcaa caactttccg aagcttggga aaactctcaa tgttcctccg
     4261 cccccggacc ccggtagggc cagcacttcc gggacaccca ttaaaaaggg cacagacgcg
     4321 agattagcct catttggaac ggagatcgcg tcttcattga caggtggtgc aacccaatgt
     4381 gctcgaaagt caccctcgga accatcaggg ccaggtgcac ctgcggggaa cgtccccgag
     4441 tgtgtgagca atgccgcact gatacaggag tggacacccg aatctggcac cacaatctcc
     4501 ccgagatccc agaataatga agaaggggga gaccattatg atgatgagct gttctctgat
     4561 gtccaagata ttaaaacagc cttggccaaa atacacgagg ataatcagaa gataatctcc
     4621 aagctagaat cactgctgtt attgaaggga gaagttgagt caattaagaa gcagatcaac
     4681 aggcaaaata tcagcatatc caccctggaa ggacacctct caagcatcat gatcgccatt
     4741 cctggacttg ggaaggatcc caacgacccc actgcagatg tcgaaatcaa tcccgacttg
     4801 aaacccatca taggcagaga ttcaggccga gcactggccg aagttctcaa gaaacccgtt
     4861 gcctcgcgac aactccaagg aatgacaaat ggacggacca gttccagagg acagctgctg
     4921 aaggaatttc agctaaagcc gatcgggaaa aagatgagct cagccgtcgg gtttgttcct
     4981 gacaccggcc ctgcatcacg cagtgtaatc cgctccatta taaaatccag ccggctagag
     5041 gaggatcgga agcgttacct gatgactctc cttgatgata tcaaaggagc caatgatctt
     5101 gccaaggacc accagatgct gatgaagata ataatgaagt agctacagct caacttacct
     5161 gccaaccgga cacctctcaa gcatcatcca tgcggacacc tctcaagcat catcagtcgg
     5221 gacacctctc aagcatcata cccaactagc gtaccatgac gtcctagtac aacctaaatc
     5281 cattataaaa aacttaggag caaagtgatt gcctcccaag ttccacaatg acagagatct
     5341 acgacttcga caagtcggca tgggacatca aagggtcgat cgctccgata caacccacca
     5401 cctacagtga tggcaggctg gtgccccagg tcagagtcat agatcctggt ctaggcgaca
     5461 ggaaggatga atgctttatg tacatgtttc tgctgggggt tgttgaggac agcgattccc
     5521 tagggcctcc aatcgggcga gcatttgggt ccctgccctt aggtgttggc agatccacag
     5581 caaagcccga aaaactcctc aaagaggcca ctgagcttga catagttgtt agacgtacag
     5641 cagggctcaa tgaaaaactg gtgttctaca acaacacccc actaactctc ctcacacctt
     5701 ggagaaaggt cctaacaaca gggagtgtct tcaacgcaaa ccaagtgtgc aatgcggtta
     5761 atctgatacc gctcgatacc ccgcagaggt tccgtgttgt ttatatgagc atcacccgtc
     5821 tttcggataa cgggtattac accgttcctg gaagaatgct ggaattcaga tcggtcaatg
     5881 cagtggcctt caacctgctg gtgaccctta ggattgacaa ggcgataggc cctgggaaga
     5941 tcatcgacaa tacagagcaa cttcctgagg caacatttat ggtccacatc gggaacttca
     6001 ggagaaagaa gagtgaagtc tactctgccg attattgcaa aatgaaaatc gaaaagatgg
     6061 gcctggtttt tgcacttggt gggatagggg gcaccagtct tcacattaga agcacaggca
     6121 aaatgagcaa gactctccat gcacaactcg ggttcaagaa gaccttatgt tacccgctga
     6181 tggatatcaa tgaagacctt aatcgattac tctggaggag cagatgcaag atagtaagaa
     6241 tccaggcagt tttgcagcca tcagttcctc aagaattccg catttacgac gacgtgatca
     6301 taaatgatga ccaaggacta ttcaaagttc tgtagaccgt agtgcccagc aatgcccgaa
     6361 aacgaccccc ctcacaatga cagccagaag gcccggacaa aaaagccccc tccgaaagac
     6421 tccacggacc aagcgagagg ccagccagca gccgacggca agcgcgaaca ccaggcggcc
     6481 ccagcacaga acagccctga cacaaggcca ccaccagcca ccccaatctg catcctcctc
     6541 gtgggacccc cgaggaccaa cccccaaggc tgcccccgat ccaaaccacc aaccgcatcc
     6601 ccaccacccc cgggaaagaa acccccagca attggaaggc ccctccccct cttcctcaac
     6661 acaagaactc cacaaccgaa ccgcacaagc gaccgaggtg acccaaccgc aggcatccga
     6721 ctccctagac agatcctctc tccccggcaa actaaacaaa acttagggcc aaggaacata
     6781 cacacccaac agaacccaga ccccggccca cggcgccgcg cccccaaccc ccgacaacca
     6841 gagggagccc ccaaccaatc ccgccggctc ccccggtgcc cacaggcagg gacaccaacc
     6901 cccgaacaga cccagcaccc aaccatcgac aatccaagac gggggggccc ccccaaaaaa
     6961 aggcccccag gggccgacag ccagcaccgc gaggaagccc acccacccca cacacgacca
     7021 cggcaaccaa accagaaccc agaccaccct gggccaccag ctcccagact cggccatcac
     7081 cccgcagaaa ggaaaggcca caacccgcgc accccagccc cgatccggcg gggagccacc
     7141 caacccgaac cagcacccaa gagcgatccc cgaaggaccc ccgaaccgca aaggacatca
     7201 gtatcccaca gcctctccaa gtcccccggt ctcctcctct tctcgaaggg accaaaagat
     7261 caatccacca cacccgacga cactcaactc cccaccccta aaggagacac cgggaatccc
     7321 agaatcaaga ctcatccaat gtccatcatg ggtctcaagg tgaacgtctc tgccatattc
     7381 atggcagtac tgttaactct ccaaacaccc accggtcaaa tccattgggg caatctctct
     7441 aagatagggg tggtaggaat aggaagtgca agctacaaag ttatgactcg ttccagccat
     7501 caatcattag tcataaaatt aatgcccaat ataactctcc tcaataactg cacgagggta
     7561 gagattgcag aatacaggag actactgaga acagttttgg aaccaattag agatgcactt
     7621 aatgcagtga cccagaatat aagaccggtt cagagtgtag cttcaagtag gagacacaag
     7681 agatttgcgg gagtagtcct ggcaggtgcg gccctaggcg ttgccacagc tgctcagata
     7741 acagccggca ttgcacttca ccagtccatg ctgaactctc aagccatcga caatctgaga
     7801 gcgagcctgg aaactactaa tcaggcaatt gaggcaatca gacaagcagg gcaggagatg
     7861 atattggctg ttcagggtgt ccaagactac atcaataatg agctgatacc gtctatgaac
     7921 caactatctt gtgatttaat cggccagaag ctcgggctca aattgctcag atactataca
     7981 gaaatcctgt cattatttgg ccccagctta cgggacccca tatctgcgga gatatctatc
     8041 caggctttga gctatgcgct tggaggagac atcaataagg tgttagaaaa gctcggatac
     8101 agtggaggtg atttactggg catcttagag agcagaggaa taaaggcccg gataactcac
     8161 gtcgacacag agtcctactt cattgtcctc agtatagcct atccgacgct gtccgagatt
     8221 aagggggtga ttgtccaccg gctagagggg gtctcgtaca acataggctc tcaagagtgg
     8281 tataccactg tgcccaagta tgttgcaacc caagggtacc ttatctcgaa ttttgatgag
     8341 tcatcgtgta ctttcatgcc agaggggact gtgtgcagcc aaaatgcctt gtacccgatg
     8401 agtcctctgc tccaagaatg cctccggggg tccaccaagt cctgtgctcg tacactcgta
     8461 tccgggtctt ttgggaaccg gttcatttta tcacaaggga acctaatagc caattgtgca
     8521 tcaatccttt gcaagtgtta cacaacagga acgatcatta atcaagaccc tgacaagatc
     8581 ctaacataca ttgctgccga tcactgcccg gtagtcgagg tgaacggcgt gaccatccaa
     8641 gtcgggagca ggaggtatcc agacgctgtg tacttgcaca gaattgacct cggtcctccc
     8701 atatcattgg agaggttgga cgtatggaca aatctgggga atgcaattgc taagttggag
     8761 gatgccaagg aattgttgga gtcatcggac cagatattga ggagtatgaa aggtttatcg
     8821 agcactagca tagtctacat cctgattgca gtgtgtcttg gagggttgat agggatcccc
     8881 gctttaatat gttgctgcag ggggcgttgt aacaaaaagg gagaacaagt tggtatgtca
     8941 agaccaggcc taaagcctga tcttacggga acatcaaaat cctatgtaag gtcgctctga
     9001 tcctctacaa ctcttgaaac acaaatgtcc cacaagtctc ctcttcgtca tcaagcaacc
     9061 accgcaccca gcatcaagcc cacctgaaat tatctccggt ttccctctgg ccgaacaata
     9121 tcggtagtta attaaaactt agggtgcaag atcatccaca atgtcaccac aacgagaccg
     9181 gataaatgcc ttctacaaag ataaccccca tcccaaggga agtaggatag tcattaacag
     9241 agaacatctt atgattgata gaccttatgt tttgctggct gttctgtttg tcatgtttct
     9301 gagcttgatc gggttgctag ccattgcagg cattagactt catcgggcag ccatctacac
     9361 cgcagagatc cataaaagcc tcagcaccaa tctagatgta actaactcaa tcgagcatca
     9421 ggtcaaggac gtgctgacac cactcttcaa aatcatcggt gatgaagtgg gcctgaggac
     9481 acctcagaga ttcactgacc tagtgaaatt catctctgac aagattaaat tccttaatcc
     9541 ggatagggag tacgacttca gagatctcac ttggtgtatc aacccgccag agagaatcaa
     9601 attggattat gatcaatact gtgcagatgt ggctgctgaa gagctcatga atgcattggt
     9661 gaactcaact ctactggaga ccagaacaac caatcagttc ctagctgtct caaagggaaa
     9721 ctgctcaggg cccactacaa tcagaggtca attctcaaac atgtcgctgt ccctgttaga
     9781 cttgtattta ggtcgaggtt acaatgtgtc atctatagtc actatgacat cccagggaat
     9841 gtatggggga acttacctag tggaaaagcc taatctgagc agcaaaaggt cagagttgtc
     9901 acaactgagc atgtaccgag tgtttgaagt aggtgttatc agaaatccgg gtttgggggc
     9961 tccggtgttc catatgacaa actatcttga gcaaccagtc agtaatgatc tcagcaactg
    10021 tatggtggct ttgggggagc tcaaactcgc agccctttgt cacggggaag attctatcac
    10081 aattccctat cagggatcag ggaaaggtgt cagcttccag ctcgtcaagc taggtgtctg
    10141 gaaatcccca accgacatgc aatcctgggt ccccttatca acggatgatc cagtgataga
    10201 caggctttac ctctcatctc acagaggtgt tatcgctgac aaccaagcaa aatgggctgt
    10261 cccgacaaca cgaacagatg acaagttgcg aatggagaca tgcttccaac aggcgtgtaa
    10321 gggtaaaatc caagcactct gcgagaatcc cgagtgggca ccattgaagg ataacaggat
    10381 tccttcatac ggggtcttgt ctgttgatct gagtctgaca gttgagctta aaatcaaaat
    10441 tgcttcggga ttcgggccat tgatcacaca cggttcaggg atggacctat acaaatccaa
    10501 ccacaacaat gtgtattggc tgactatccc gccaatgaag aacctagcct taggtgtaat
    10561 caacacattg gagtggatac cgagattcaa ggttagtccc tacctcttca ctgtcccaat
    10621 taaggaagca ggcggagact gccatgcccc aacataccta cctgcggagg tggatggtga
    10681 tgtcaaactc agttccaatc tggtgattct acctggtcaa gatctccaat atgttttggc
    10741 aacctacgat acttccaggg ttgaacatgc tgtggtttat tacgtttaca gcccaagccg
    10801 ctcattttct tacttttatc cttttaggtt gcctataaag ggggtcccca tcgaattaca
    10861 agtggaatgc ttcacatggg accaaaaact ctggtgccgt cacttctgtg tgcttgcgga
    10921 ctcagaatct ggtggacata tcactcactc tgggatggtg ggcatgggag tcagctgcac
    10981 agtcacccgg gaagatggaa ccaatcgcag atagggctgc tagtgaacca atcacatgat
    11041 gtcacccaga catcaggcat acccactagt gtgaaataga catcagaatt aagaaaaacg
    11101 tagggtccaa gtggttcccc gttatggact cgctatctgt caaccagatc ttataccctg
    11161 aagttcacct agatagcccg atagttacca ataagatagt agccatcctg gagtatgctc
    11221 gagtccctca cgcttacagc ctggaggacc ctacactgtg tcagaacatc aagcaccgcc
    11281 taaaaaacgg attttccaac caaatgatta taaacaatgt ggaagttggg aatgtcatca
    11341 agtccaagct taggagttat ccggcccact ctcatattcc atatccaaat tgtaatcagg
    11401 atttatttaa catagaagac aaagagtcaa cgaggaagat ccgtgaactc ctcaaaaagg
    11461 ggaattcgct gtactccaaa gtcagtgata aggttttcca atgcttaagg gacactaact
    11521 cacggcttgg cctaggctcc gaattgaggg aggacatcaa ggagaaagtt attaacttgg
    11581 gagtttacat gcacagctcc cagtggtttg agccctttct gttttggttt acagtcaaga
    11641 ctgagatgag gtcagtgatt aaatcacaaa cccatacttg ccataggagg agacacacac
    11701 ctgtattctt cactggtagt tcagttgagt tgctaatctc tcgtgacctt gttgctataa
    11761 tcagtaaaga gtctcaacat gtatattacc tgacatttga actggttttg atgtattgtg
    11821 atgtcataga ggggaggtta atgacagaga ccgctatgac tattgatgct aggtatacag
    11881 agcttctagg aagagtcaga tacatgtgga aactgataga tggtttcttc cctgcactcg
    11941 ggaatccaac ttatcaaatt gtagccatgc tggagcctct ttcacttgct tacctgcagc
    12001 tgagggatat aacagtagaa ctcagaggtg ctttccttaa ccactgcttt actgaaatac
    12061 atgatgttct tgaccaaaac gggttttctg atgaaggtac ttatcatgag ttaattgaag
    12121 ctctagatta cattttcata actgatgaca tacatctgac aggggagatt ttctcatttt
    12181 tcagaagttt cggccacccc agacttgaag cagtaacggc tgctgaaaat gttaggaaat
    12241 acatgaatca gcctaaagtc attgtgtatg agactctgat gaaaggtcat gccatatttt
    12301 gtggaatcat aatcaacggc tatcgtgaca ggcacggagg cagttggcca ccgctgaccc
    12361 tccccctgca tgctgcagac acaatccgga atgctcaagc ttcaggtgat gggttaacac
    12421 atgagcagtg cgttgataac tggaaatctt ttgctggagt gaaatttggc tgctttatgc
    12481 ctcttagcct ggatagtgat ctgacaatgt acctaaagga caaggcactt gctgctctcc
    12541 aaagggaatg ggattcagtt tacccgaaag agttcctgcg ttacgaccct cccaagggaa
    12601 ccgggtcacg gaggcttgta gatgttttcc ttaatgattc gagctttgac ccatatgatg
    12661 tgataatgta tgttgtaagt ggagcttacc tccatgaccc tgagttcaac ctgtcttaca
    12721 gcctgaaaga aaaggagatc aaggaaacag gtagactttt tgctaaaatg acttacaaaa
    12781 tgagggcatg ccaagtgatt gctgaaaatc taatctcaaa cgggattggc aaatatttta
    12841 aggacaatgg gatggccaag gatgagcacg atttgactaa ggcactccac actctagctg
    12901 tctcaggagt ccccaaagat ctcaaagaaa gtcacagggg ggggccagtc ttaaaaacct
    12961 actcccgaag cccagtccac acaagtacca ggaacgtgag agcagcaaaa gggtttatag
    13021 ggttccctca agtaattcgg caggaccaag acactgatca tccggagaat atggaagctt
    13081 acgagacagt cagtgcattt atcacgactg atctcaagaa gtactgcctt aattggagat
    13141 atgagaccat cagcttgttt gcacagaggc taaatgagat ttacggattg ccctcatttt
    13201 tccagtggct gcataagagg cttgagacct ctgtcctgta tgtaagtgac cctcattgcc
    13261 cccccgacct tgacgcccat atcccgttat ataaagtccc caatgatcaa atcttcatta
    13321 agtaccctat gggaggtata gaagggtatt gtcagaagct gtggaccatc agcaccattc
    13381 cctatctata cctggctgct tatgagagcg gagtaaggat tgcttcgtta gtgcaagggg
    13441 acaatcagac catagccgta acaaaaaggg tacccagcac atggccctac aaccttaaga
    13501 aacgggaagc tgctagagta actagagatt actttgtaat tcttaggcaa aggctacatg
    13561 atattggcca tcacctcaag gcaaatgaga caattgtttc atcacatttt tttgtctatt
    13621 caaaaggaat atattatgat gggctacttg tgtcccaatc actcaagagc atcgcaagat
    13681 gtgtattctg gtcagagact atagttgatg aaacaagggc agcatgcagt aatattgcta
    13741 caacaatggc taaaagcatc gagagaggtt atgaccgtta ccttgcatat tccctgaacg
    13801 tcctaaaagt gatacagcaa attctgatct ctcttggctt cacaatcaat tcaaccatga
    13861 cccgggatgt agtcataccc ctcctcacaa acaacgacct cttaataagg atggcactgt
    13921 tgcccgctcc tattgggggg atgaattatc tgaatatgag caggctgttt gtcagaaaca
    13981 tcggtgatcc agtaacatca tcaattgctg atctcaagag aatgattctc gcctcactaa
    14041 tgcctgaaga gaccctccat caggtaatga cacaacaacc gggggactct tcattcctag
    14101 actgggctag cgacccttac tcagcaaatc ttgtatgtgt ccagagcatc actagactcc
    14161 tcaagaacat aactgcaagg tttgtcctga tccatagtcc aaacccaatg ttaaaaggat
    14221 tattccatga tgacagtaaa gaagaggacg agggactggc ggcattcctc atggacaggc
    14281 atattatagt acctagggca gctcatgaaa tcctggatca tagtgtcaca ggggcaagag
    14341 agtctattgc aggcatgctg gataccacaa aaggcttgat tcgagccagc atgaggaagg
    14401 ggggtttaac ctctcgagtg ataaccagat tgtccaatta tgactatgaa caattcagag
    14461 cagggatggt gctattgaca ggaagaaaga gaaatgtcct cattgacaaa gagtcatgtt
    14521 cagtgcagct ggcgagagct ctaagaagcc atatgtgggc gaggctagct cgaggacggc
    14581 ctatttacgg ccttgaggtc cctgatgtac tagaatctat gcgaggccac cttattcggc
    14641 gtcatgagac atgtgtcatc tgcgagtgtg gatcagtcaa ctacggatgg ttttttgtcc
    14701 cctcgggttg ccaactggat gatattgaca aggaaacatc atccttgaga gtcccatata
    14761 ttggttctac cactgatgag agaacagaca tgaagcttgc cttcgtaaga gccccaagtc
    14821 gatccttgcg atctgctgtt agaatagcaa cagtgtactc atgggcttac ggtgatgatg
    14881 atagctcttg gaacgaagcc tggttgttgg ctaggcaaag ggccaatgtg agcctggagg
    14941 agctaagggt gatcactccc atctcaactt cgactaattt agcgcatagg ttgagggatc
    15001 gtagcactca agtgaaatac tcaggtacat cccttgtccg agtggcgagg tataccacaa
    15061 tctccaacga caatctctca tttgtcatat cagataagaa ggttgatact aactttatat
    15121 accaacaagg aatgcttcta gggttgggtg ttttagaaac attgtttcga ctcgagaaag
    15181 ataccggatc atctaacacg gtattacatc ttcacgtcga aacagattgt tgcgtgatcc
    15241 cgatgataga tcatcccagg atacccagct cccgcaagct agagctgagg gcagagctat
    15301 gtaccaaccc attgatatat gataatgcac ctttaattga cagagatgca acaaggctat
    15361 acacccagag ccataggagg caccttgtgg aatttgttac atggtccaca ccccaactat
    15421 atcacatttt agctaagtcc acagcactat ctatgattga cctggtaaca aaatttgaga
    15481 aggaccatat gaatgaaatt tcagctctca taggggatga cgatatcaat agtttcataa
    15541 ctgagtttct gctcatagag ccaagattat tcactatcta cttgggccag tgtgcggcca
    15601 tcaattgggc atttgatgta cattatcata gaccatcagg gaaatatcag atgggtgagc
    15661 tgttgtcatc gttcctttct agaatgagca aaggagtgtt taaggtgctt gtcaatgctc
    15721 taagccaccc aaagatctac aagaaattct ggcattgtgg tattatagag cctatccatg
    15781 gtccttcact tgatgctcaa aacttgcaca caactgtgtg caacatggtt tacacatgct
    15841 atatgaccta cctcgacctg ttgttgaatg aagagttaga agagttcaca tttctcttgt
    15901 gtgaaagcga cgaggatgta gtaccggaca gattcgacaa catccaggca aaacacttat
    15961 gtgttctggc agatttgtac tgtcaaccag ggacctgccc accaattcaa ggtctaagac
    16021 cggtagagaa atgtgcagtt ctaaccgacc atatcaaggc agaggctatg ttatctccag
    16081 caggatcttc gtggaacata aatccaatta ttgtagacca ttactcatgc tccctgactt
    16141 atctccggcg aggatcgatc aaacagataa gattgagagt tgatccagga ttcattttcg
    16201 acgccctcgc tgaggtaaat gtcagtcagc caaagatcgg cagcaacaac atctcaaata
    16261 tgagcatcaa ggctttcaga cccccacacg atgatgttgc aaaattgctc aaagatatca
    16321 acacaagcaa gcacaatctt cccatttcag ggggcaatct cgccaattat gaaatccatg
    16381 ctttccgcag aatcgggttg aactcatctg cttgctacaa agctgttgag atatcaacat
    16441 taattaggag atgccttgag ccaggggagg acggcttgtt cttgggtgag ggatcgggtt
    16501 ctatgttgat cacttataag gagatactta aactaagcaa gtgcttctat aatagtgggg
    16561 tttccgccaa ttctagatct ggtcaaaggg aattagcacc ctatccctcc gaagttggcc
    16621 ttgtcgaaca cagaatggga gtaggtaata ttgtcaaagt gctctttaac gggaggcccg
    16681 aagtcacgtg ggtaggcagt gtagattgct tcaatttcat agttagtaat atccctacct
    16741 ctagtgtggg gtttatccat tcagatatag agaccttgcc tgacaaagat actatagaga
    16801 agctagagga attggcagcc atcttatcga tggctctgct cctgggcaaa ataggatcaa
    16861 tactggtgat taagcttatg cctttcagcg gggattttgt tcagggattt ataagttatg
    16921 tagggtctca ttatagagaa gtgaaccttg tataccctag atacagcaac ttcatatcta
    16981 ctgaatctta tttggttatg acagatctca aggctaaccg gctaatgaat cctgaaaaga
    17041 ttaagcagca gataattgaa tcatctgtga ggacttcacc tggacttata ggtcacatcc
    17101 tatccattaa gcaactaagc tgcatacaag caattgtggg agacgcagtt agtagaggtg
    17161 atatcaatcc tactctgaaa aaacttacac ctatagagca ggtgctgatc aattgcgggt
    17221 tggcaattaa cggacctaag ctgtgcaaag aattgatcca ccatgatgtt gcctcagggc
    17281 aagatggatt gcttaattct atactcatcc tctacaggga gttggcaaga ttcaaagaca
    17341 accaaagaag tcaacaaggg atgttccacg cttaccccgt attggtaagt agcaggcaac
    17401 gagaacttat atctaggatc acccgcaaat tttgggggca cattcttctt tactccggga
    17461 acagaaagtt gataaataag tttatccaga atctcaagtc cggctatctg atactagact
    17521 tacaccagaa tatcttcgtt aagaatctat ccaagtcaga gaaacagatt attatgacgg
    17581 ggggtttgaa acgtgagtgg gtttttaagg taacagtcaa ggagaccaaa gaatggtata
    17641 agttagtcgg atacagtgcc ctgattaagg actaattggt tgaactccgg aaccctaatc
    17701 ctgccctagg tggttaggca ttatttgcaa tatattaaag aaaactttga aaatacgaag
    17761 tttctattcc cagctttgtc tggtggccgg catggtccca gcctcctcgc tggcgctggc
    17821 tgggcaacat tccgagggga ccgtcccctc ggtaatggcg aatgggacgc ggccgatccg
    17881 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
    17941 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
    18001 atatccggat gcggccgcag gtacccagct tttgttccct ttagtgaggg ttaatttcga
    18061 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    18121 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    18181 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    18241 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    18301 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    18361 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    18421 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    18481 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    18541 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    18601 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    18661 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    18721 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    18781 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    18841 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    18901 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    18961 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    19021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    19081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    19141 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    19201 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    19261 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    19321 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    19381 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    19441 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    19501 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    19561 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    19621 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    19681 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    19741 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    19801 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    19861 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    19921 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    19981 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    20041 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    20101 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    20161 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    20221 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
    20281 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
    20341 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    20401 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    20461 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    20521 agggagcccc cgatttagag cttgacgggg aaagccggcc atttaggcca tagggcgctg
    20581 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    20641 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    20701 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    20761 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
    20821 gactcactat a
//
