LOCUS       sequence-91099-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: tdEos-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..89
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
       61 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atgagtgcga
      121 ttaagccaga catgaagatc aacctccgta tggaaggcaa cgtaaacggg caccactttg
      181 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
      241 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg
      301 gcaacagggt attcgccgaa tatccagacc acatacaaga ctattttaag cagtcgtttc
      361 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca
      421 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt cacggtgtaa
      481 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
      541 agaaaatgta tgtgcgtgat ggagtgctga ctggtgatat tcgcatggct ttgttgcttg
      601 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
      661 tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag
      721 attacaacaa ggttaagctg tatgagcatg ctg
//
