LOCUS       sequence-91115-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector (AmpR, high copy, blue/white selection, M13 
            IR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pUC21
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(13..29)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..29)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(157..173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(170..192)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    252..273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(390..407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(641..660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaattgggga tcgatccact agttctagag cggccgccac ggcgatatcg gatccatatg
       61 acgtcgacgc gtctgcagaa gcttcgaatt cgagctcccg ggtaccatgg catgcatcga
      121 tagatctcga ggcctcggac tagtggcgta atcatggtca tagctgtttc ctgtgtgaaa
      181 ttgttatccg ctcacaattc cacacaacat acgagccgcg gaagcataaa gtgtaaagcc
      241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      301 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      361 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      721 gcctttctcc cttcggg
//
