LOCUS       sequence-91116-e         366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Norrander J, Kempe T, Messing J
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis.
  JOURNAL   Gene. 1983 Dec;26(1):101-6.
  PUBMED    6323249
REFERENCE   2  (bases 1 to 366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1983-12"; volume: "26(1)"; pages: "101-6"
COMMENT     Sequence Label: pUC12
FEATURES             Location/Qualifiers
     source          1..366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(33..49)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(33..49)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(46..68)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    57..73
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(264..281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggatccccgg gcgagctcga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
       61 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      181 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      241 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      301 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      361 aacgca
//
