LOCUS       sequence-91118-e         201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Norrander J, Kempe T, Messing J
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis.
  JOURNAL   Gene. 1983 Dec;26(1):101-6.
  PUBMED    6323249
REFERENCE   2  (bases 1 to 201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1983-12"; volume: "26(1)"; pages: "101-6"
COMMENT     Sequence Label: pUC19
FEATURES             Location/Qualifiers
     source          1..201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(45..61)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(45..61)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(58..80)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    69..85
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(93..99,100..117,118..123))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    138..159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
       61 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      181 gctttccagt cgggaaacct g
//
