LOCUS       sequence-91146-e        6883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human apoptosis inhibitor XIAP from CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6883)
  TITLE     pSil-ETN-ires-GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSil-XIAP
FEATURES             Location/Qualifiers
     source          1..6883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..660
                     /label=chicken beta-actin promoter
     intron          661..1669
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1593..1615
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1677..1696
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3363..3382)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3428..3483
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3482..3501)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3844..3860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3844..3860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3857..3879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3868..3884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3892..3898,3899..3916,3917..3922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3937..3958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4075..4092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4246..4834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4326..4345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5304..5682)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     5629..5648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5867..5971)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6004..6317
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     6236..6256
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     6329..6345
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6330..6346
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        6386..6765
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      421 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      481 gatgggggcg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggagct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
      961 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
     1021 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     1081 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
     1141 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
     1201 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     1261 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg
     1321 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     1381 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1441 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     1501 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     1561 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1621 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1681 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgcggccgt ctcaggccga
     1741 attcatcgat tgtttaaacg cggccaagat gacttttaac agttttgaag gatctaaaac
     1801 ttgtgtacct gcagacatca ataaggaaga agaatttgta gaagagttta atagattaaa
     1861 aacttttgct aattttccaa gtggtagtcc tgtttcagca tcaacactgg cacgagcagg
     1921 gtttctttat actggtgaag gagataccgt gcggtgcttt agttgtcatg cagctgtaga
     1981 tagatggcaa tatggagact cagcagttgg aagacacagg aaagtatccc caaattgcag
     2041 atttatcaac ggcttttatc ttgaaaatag tgccacgcag tctacaaatt ctggtatcca
     2101 gaatggtcag tacaaagttg aaaactatct gggaagcaga gatcattttg ccttagacag
     2161 gccatctgag acacatgcag actatctttt gagaactggg caggttgtag atatatcaga
     2221 caccatatac ccgaggaacc ctgccatgta tagtgaagaa gctagattaa agtcctttca
     2281 gaactggcca gactatgctc acctaacccc aagagagtta gcaagtgctg gactctacta
     2341 cacaggtatt ggtgaccaag tgcagtgctt ttgttgtggt ggaaaactga aaaattggga
     2401 accttgtgat cgtgcctggt cagaacacag gcgacacttt cctaattgct tctttgtttt
     2461 gggccggaat cttaatattc gaagtgaatc tgatgctgtg agttctgata ggaatttccc
     2521 aaattcaaca aatcttccaa gaaatccatc catggcagat tatgaagcac ggatctttac
     2581 ttttgggaca tggatatact cagttaacaa ggagcagctt gcaagagctg gattttatgc
     2641 tttaggtgaa ggtgataaag taaagtgctt tcactgtgga ggagggctaa ctgattggaa
     2701 gcccagtgaa gacccttggg aacaacatgc taaatggtat ccagggtgca aatatctgtt
     2761 agaacagaag ggacaagaat atataaacaa tattcattta actcattcac ttgaggagtg
     2821 tctggtaaga actactgaga aaacaccatc actaactaga agaattgatg ataccatctt
     2881 ccaaaatcct atggtacaag aagctatacg aatggggttc agtttcaagg acattaagaa
     2941 aataatggag gaaaaaattc agatatctgg gagcaactat aaatcacttg aggttctggt
     3001 tgcagatcta gtgaatgctc agaaagacag tatgcaagat gagtcaagtc agacttcatt
     3061 acagaaagag attagtactg aagagcagct aaggcgcctg caagaggaga agctttgcaa
     3121 aatctgtatg gatagaaata ttgctatcgt ttttgttcct tgtggacatc tagtcacttg
     3181 taaacaatgt gctgaagcag ttgacaagtg tcccatgtgc tacacagtca ttactttcaa
     3241 gcaaaaaatt tttatgtctt aatctaactc tatagtaggc atgttatgtt gttcttatta
     3301 ccctgattga atgtgtgatg tgaactgact ttaagtaatc aggattgaat tccattagcg
     3361 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     3421 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     3481 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     3541 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     3601 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     3661 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     3721 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     3781 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     3841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3901 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4681 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5221 tgcaacttta tccgcctcca tccagttcta ttaattgttg ccgggaagct agagtaagta
     5281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
     6001 cgagatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag
     6061 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
     6121 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
     6181 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
     6241 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct
     6301 tggagaaaag ccttgtttgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
     6361 gaattcctgc agcccggggg atctcgacat tgattattga ctagttatta atagtaatca
     6421 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6481 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6541 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6601 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6661 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6721 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     6781 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     6841 attttttaat tattttgtgc agcgatgggg gcgggggggg ggg
//
