LOCUS       sequence-91149-e        2367 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, 
            under EF1a promoter, in AAV virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2367)
  TITLE     pAAV-FLEX-C-RG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-FLEX-C-RG
FEATURES             Location/Qualifiers
     source          1..2367
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..714)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(43..64)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(304..323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     651..672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             730..789
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
       61 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      181 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
      241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      421 ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
      481 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      601 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggctagc
      721 ggtagtggtc aatgtactaa ctacgctttg ttgaaactcg ctggcgatgt tgaaagtaac
      781 cccggtcctt ccggagtgcc ccaggccctg ctgttcgtgc ccctgctggt gttccccctg
      841 tgcttcggca agttccccat ctacaccatc cccgacaagc tgggcccctg gagccccatc
      901 gacatccacc acctgagctg ccccaacaac ctggtggtgg aggacgaggg ctgcaccaac
      961 ctgagcggct tcagctacat ggagctgaag gtgggctaca tcctggccat caaggtgaac
     1021 ggcttcacct gcaccggcgt ggtgaccgag gccgagacct acaccaactt cgtgggctac
     1081 gtgaccacca ccttcaagag aaagcacttc agacccaccc ccgacgcctg cagagccgcc
     1141 tacaactgga agatggccgg cgaccccaga tacgaggaga gcctgcacaa cccctacccc
     1201 gactacagat ggctgagaac cgtgaagacc accaaggaga gcctggtgat catcagcccc
     1261 agcgtggccg acctggaccc ctacgacaga agcctgcaca gcagagtgtt ccccagcggc
     1321 aagtgcagcg gcgtggccgt gagcagcacc tactgcagca ccaaccacga ctacaccatc
     1381 tggatgcccg agaaccccag actgggcatg agctgcgaca tcttcaccaa cagcagaggc
     1441 aagagagcca gcaagggcag cgagacctgc ggcttcgtgg acgagagagg cctgtacaag
     1501 agcctgaagg gcgcctgcaa gctgaagctg tgcggcgtgc tgggcctgag actgatggac
     1561 ggcacctggg tgagcatgca gaccagcaac gagaccaagt ggtgcccccc cgacaagctg
     1621 gtgaacctgc acgacttcag aagcgacgag atcgagcacc tggtggtgga ggagctggtg
     1681 agaaagagag aggagtgcct ggacgccctg gagagcatca tgaccaccaa gagcgtgagc
     1741 ttcagaagac tgagccacct gagaaagctg gtgcccggct tcggcaaggc ctacaccatc
     1801 ttcaacaaga ccctgatgga ggccgacgcc cactacaaga gcgtgagaac ctggaacgag
     1861 atcctgccca gcaagggctg cctgagagtg ggcggcagat gccaccccca cgtgaacggc
     1921 gtgttcttca acggcatcat cctgggcccc gacggcaacg tgctgatccc cgagatgcag
     1981 agcagcctgc tgcagcagca catggagctg ctggagagca gcgtgatccc cctggtgcac
     2041 cccctggccg accccagcac cgtgttcaag gacggcgacg aggccgagga cttcgtggag
     2101 gtgcacctgc ccgacgtgca caaccaggtg agcggcgtgg acctgggcct gcccaactgg
     2161 ggcaagtacg tgctgctgag cgccggcgcc ctgaccgccc tgatgctgat catcttcctg
     2221 atgacctgct gcagaagagt gaacagaagc gagcccaccc agcacaacct gagaggcacc
     2281 ggcagagagg tgagcgtgac cccccagagc ggcaagatca tcagcagctg ggagagccac
     2341 aagagcggcg gcgagaccag actgtga
//
