LOCUS       sequence-91154-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector, integration at amyE, ampr, cmr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS1C
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    646..667
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    768..789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(804..809,810..827,828..834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    842..858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             876..887
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttctccagtc ttcacatcgg tttgaaagga ggaagcggaa gaatgaagta agagggattt
       61 ttgactccga agtaagtctt caaaaaatca aataaggagt gtcaagaatg tttgcaaaac
      121 gattcaaaac ctctttactg ccgttattcg ctggattttt attgctgttt catttggttc
      181 tggcaggacc ggcggctgcg agtgctgaaa cggcgaacaa atcgaatgag cttacagcac
      241 cgtcgatcaa aagcggaacc attcttcatg catggaattg gtcgttcaat acgttaaaac
      301 acaatatgaa ggatattcat gatgcaggat atacagccat tcagacatct ccgattaacc
      361 aagtaaagga agggaatcaa ggagataaaa gcatgtcgaa ctggtactgg ctgtatcagc
      421 cgacatcgta tcaaattggc aaccgttact taggtactga acaagaattt aaagaaatgt
      481 gtgcagccgc tgaagaatat ggcataaagg tcattgttga cgcggtcatc aatcatacca
      541 ccagtgatta tgccgcgatt tccaatgagg ttaagagtat tccaaactgg acacatggaa
      601 acacacaaat taaaaactgg tctgatcgga tcctagaagc ttatcgaatt cgcggccgct
      661 tctagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      721 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      781 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      841 attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt
      901 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
      961 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
     1021 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttggga
//
