LOCUS       sequence-91157-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector, integration at thrC, ampr, specr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS4S
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(29..100)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(145..822)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             829..840
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    858..874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(882..888,889..906,907..912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    927..948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1049..1070
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac
       61 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtagcga
      121 tctacactag cactatcagc gttattaagc accggtggag tgacgacctt cagcacgttc
      181 gtactgttca acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt
      241 tttgtaagca cccggcagct gaaccggttt tttagccatg taggtggttt taacttcagc
      301 gtcgtagtga ccaccgtctt tcagtttcag acgcattttg atttcacctt tcagagcacc
      361 gtcttccggg tacatacgtt cggtggaagc ttcccaaccc atggtttttt tctgcataac
      421 cggaccatcg gacgggaagt tggtaccacg cagtttaact ttgtagatga actcaccgtc
      481 ttgcagggag gagtcctggg taacggtaac aacaccaccg tcttcgaagt tcataacacg
      541 ttcccatttg aaaccttccg ggaaggacag tttcaggtag tccgggatgt cagccgggtg
      601 tttaacgtaa gctttggaac cgtactggaa ctgcggggac aggatgtccc aagcgaacgg
      661 cagcggacca cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc
      721 ttcaccttca ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt
      781 gaaacgcatg aactctttga taacgtcttc ggaggaagcc atctagtatt tctcctcttt
      841 ctctagtatg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1021 cgcgcgggga gaggcggttt gcgtattgct ctagaagcgg ccgcgaattc
//
