LOCUS       sequence-91160-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4a-C1
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..720
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     misc_feature    721..786
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagtgc gattaagcca
       61 gacatgagga tcaaactccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac
      121 ggagatggta caggcaagcc ttatgaggga aaacagacca tggatcttga agtcaaagag
      181 ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg
      241 gtattcgtta aatatccaga caacatacaa gactatttta agcagtcgtt tcctaagggg
      301 tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgctatgc cagaaacgac
      361 ataacaatgg aaggggacac tttctataat aaagttcgat tttatggtac caactttccc
      421 gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg
      481 tatgtgcgtg atggagtgct gacgggtgat attcatatgg ctttgttgct tgaaggaaat
      541 gcccattacc gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta
      601 ccaggctacc actttgtgga ccacgccatt gagattttaa gccatgacaa agattacaac
      661 aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga
      721 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
      781 ggatccaccg g
//
