LOCUS       sequence-91161-e        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacZ-reporter vector, integration at amyE, ampr, cmr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS1ClacZ
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(204..308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     376..394
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
       61 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      301 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
      361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attaacaaaa
      421 ttctccagtc ttcacatcgg tttgaaagga ggaagcggaa gaatgaagta agagggattt
      481 ttgactccga agtaagtctt caaaaaatca aataaggagt gtcaagaatg tttgcaaaac
      541 gattcaaaac ctctttactg ccgttattcg ctggattttt attgctgttt catttggttc
      601 tggcaggacc ggcggctgcg agtgctgaaa cggcgaacaa atcgaatgag cttacagcac
      661 cgtcgatcaa aagcggaacc attcttcatg catggaattg gtcgttcaat acgttaaaac
      721 acaatatgaa ggatattcat gatgcaggat atacagccat tcagacatct ccgattaacc
      781 aagtaaagga agggaatcaa ggagataaaa gcatgtcgaa ctggtactgg ctgtatcagc
      841 cgacatcgta tcaaattggc aaccgttact taggtactga acaagaattt aaagaaatgt
      901 gtgcagccgc tgaagaatat ggcataaagg tcattgttga cgcggtcatc aatcatacca
      961 ccagtgatta tgccgcgatt tccaatgagg ttaagagtat tccaaactgg acacatggaa
     1021 acacacaaat taaaaactgg tctgatcgat gggatgtcac gcagaattaa tagtacataa
     1081 tggatttcct tacgcgaa
//
