LOCUS       sequence-91162-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacZ-reporter vector, integration at amyE, ampr, cmr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS1ClacZ
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    229..249
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(256..283)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(299..370)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa
       61 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      121 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccggtcga cccagtcacg
      181 ttgtaaaacg accatcgtca tgtctccttt tttatgtact gtggatccct gcagcggccg
      241 ctactagtat ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga
      301 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
      361 gttttatttg atgcctggct ctagtagcga tctacactag cactatcagc gttattaagc
      421 accggtggag tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt
      481 gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaaccggttt
      541 tttagccatg taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag
      601 acgcattttg atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc
      661 ttcccaaccc atggtttttt tctgcataac cggaccatcg gacgggaagt tggtaccacg
      721 cagtttaact ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac
      781 aacaccaccg tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag
      841 tttcaggtag tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa
      901 ctgcggggac aggatgt
//
