LOCUS       sequence-91164-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 gctgatcaag gagaacatgc acatgaagct gtacatggaa ggcaccgtga acaaccacca
      181 cttcaagtgc accaccgaag gggagggcaa gccctacgag ggcacccaga cccagaggat
      241 taaggtggtg gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat
      301 gtacgggagc aagaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc
      361 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac
      421 cgttacccag gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg
      481 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccac
      541 caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa
      601 gctcgtgggc gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc
      661 cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa
      721 ggaggccgac aatgagacct acgtc
//
