LOCUS       sequence-91172-e        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Very strong constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: Pveg
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(138..195)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    196..216
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    454..475
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      478..521
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(525..543)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(771..790)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
      121 tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg
      181 cccttttttg ccggactgca gcggccgcta ctagtaacta catttattgt acaacacgag
      241 cccatttttg tcaaataaaa tttaaattat atcaacgtta ataagacgtt gtcaataaaa
      301 ttattttgac aaaattctat gattcctcaa ttatgttccg caagtttcgg taaattatgt
      361 atcattatgt aaaataaaaa acccgcatag ctctcctatg caggttttca actgttaatt
      421 ggacttataa ggcataccaa ttctcagaac tccctctaga agcggccgcg aattccagaa
      481 atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg tgatacgcct
      541 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcactcgagt
      601 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
      661 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      721 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      781 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      841 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      901 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
      961 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1021 ggagtgaata ccacgacgat ttccggcagt ttctac
//
