LOCUS       sequence-91173-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-C1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..735
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcga gctgattaag
       61 gagaacatgc ccatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
      121 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtcgtc
      181 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
      241 agaaccttca tcaagcaccc tccgggcatc cccgacttct ttaagcagtc cttccctgag
      301 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
      361 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca aggttagagg ggtgaacttc
      421 ccagccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc caccgagacg
      481 atgtaccccg ctgacggcgg cctggaaggc gcatgtgaca tggccctgaa gctcgtgggc
      541 gggggccacc tgatctgcaa ccttgagacc acatacagat ccaagaaacc cgctacgaac
      601 ctcaagatgc ccggcgtcta caacgtggac cacagactgg aaagaatcaa ggaggccgac
      661 gatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactctac tggtggcgct
      721 ggtgatggag gtaaaggtgg aggaggttcc ggactcagat ctcgagctca agcttcgaa
//
