LOCUS       sequence-91175-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Very strong constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: Pveg
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(35..78)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    81..102
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    340..360
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      361..418
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(442..459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(693..712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
       61 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agggagttct gagaattggt
      121 atgccttata agtccaatta acagttgaaa acctgcatag gagagctatg cgggtttttt
      181 attttacata atgatacata atttaccgaa acttgcggaa cataattgag gaatcataga
      241 attttgtcaa aataatttta ttgacaacgt cttattaacg ttgatataat ttaaatttta
      301 tttgacaaaa atgggctcgt gttgtacaat aaatgtagtt actagtagcg gccgctgcag
      361 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
      421 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      601 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1081 gaagcc
//
