LOCUS       sequence-91185-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UTYC-c613
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..89)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(90..119)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(120..140)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctgattat gatcctctag tacttctcga caagcttgtc gacggagctc gaattcggat
       61 cctcacttat cgtcgtcatc cttgtaatca tggtggtgat ggtgatggtg atgatggtgc
      121 gattggaagt agaggttctc tgcagatgag gatgataatg aaagagctag tgtaaattga
      181 tcataaactt ggattaggtc ctccattttg tactgttcga gcaccacaaa attttccaaa
      241 cttttgcttg tttttcgtgc acaatcatgg caatgtacta tgtaggtttt ttgagtattg
      301 ctttcattag tgacaaaaag cagattaaaa acctccacct cacaaatgct acagtaatga
      361 gctggttcat catttgtccg cccatgccat ataacctctt ttcctgctgc aacaagagct
      421 tctctcaatg tctgatattg cttcagaatt ttcaaaagac aatacttaat catttcaaaa
      481 agctttggat ctgagacttt gatatttcgt gccatattcc aggaaagatg caccatgggt
      541 actggtgact tcacactttt caatttgttc cattcatacc gttccactgc caatttatac
      601 tggcaggctg taagtggacc aacattccag gcaatgttat tgcaccagcc aacagcttga
      661 acccaatgca cagtgcctgc atttatccag accaaatctc caggtcgctg aataaatcta
      721 tacacaggga catttgcttc ataaagatct tcaaggttgg gccaccaaga actcattaaa
      781 aaattcaaat tatttttttc acagaagtca ttcagaacac cccaataatc ttcaggtaca
      841 acaaaccatt cacaatctcc tggaccaata tttatgttaa cagagcagaa gttgttattt
      901 tcttggtgac ctggtgtccg actccctgga actttcatat acagttgtac tgtattcatg
      961 cccagaatgg tatgcccaac atgggttaga agatttcctg ctga
//
