LOCUS       sequence-91191-e         563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli genetic modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pMDICI
FEATURES             Location/Qualifiers
     source          1..563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(61..108)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cccggcgatc ctctggagat tattagggat aacagggtaa tgtgaggctg gagctgcttc
       61 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcat ttaaatggcg
      121 cgccttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      181 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      241 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      301 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
      361 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      421 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
      481 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
      541 gctcaccgtc tttcattgcc ata
//
