LOCUS       sequence-91194-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli genetic modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pMDISI
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(56..102)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ggcaagtgga cgattattag ggataacagg gtaatgtgta ggctggagct gcttcgaagt
       61 tcctatactt tctagagaat aggaacttcg gaataggaac ttatgagctc agccaatcga
      121 ctggcgagcg gcatcttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
      181 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
      241 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
      301 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
      361 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
      421 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
      481 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
      541 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
      601 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
      661 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaagg
      721 tcgttgatca aagctcgccg cgttgttttc atcaagcctt acggtcaccg taaccagcaa
      781 atcaatatca ctgtgtggct tca
//
